首页|基于电子鼻和HS-SPME-GC-MS比较不同空间层次浓香型原酒挥发性风味成分

基于电子鼻和HS-SPME-GC-MS比较不同空间层次浓香型原酒挥发性风味成分

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采用电子鼻结合顶空固相微萃取-气相色谱-质谱联用(HS-SPME-GC-MS)技术比较不同空间层次的浓香型原酒挥发性风味物质,利用偏最小二乘法-判别分析(PLS-DA)模型筛选上下层浓香型原酒关键差异风味物质,并对电子鼻结果进行主成分分析(PCA)和线性判别分析(LDA)。结果表明,上下层浓香型原酒中共检测出76种挥发性风味化合物,包括酯类39种、酸类9种、芳香类9种、醇类8种、酮类4种、醛类3种、呋喃类3种及其他类1种,且下层浓香型原酒中酯类、酸类、缩醛类、有机酸等总含量均显著高于上层浓香型原酒(P<0。05)。其中,乙酸异戊酯、乙酸乙酯、2-己酮、己醛、丁酸、3-甲基丁酸、己酸乙酯等13种挥发性风味化合物是上下层浓香型原酒的关键差异风味物质。此外,电子鼻结果可以通过主成分分析(PCA)和线性判别分析(LDA)对上下层原酒进行较好区分,可以辅助HS-SPME-GC-MS对不同空间层次浓香型原酒中挥发性风味成分进行分析,可为保障浓香型原酒质量稳定提供一定理论依据。
Comparison of volatile flavor components of original strong-flavor Baijiu with different spatial levels based on electronic nose and HS-SPME-GC-MS
The volatile flavor compounds of original strong-flavor(Nongxiangxing)Baijiu with different spatial levels were compared by electronic nose combined with headspace solid-phase microextraction-gas chromatography-mass spectrometry(HS-SPME-GC-MS)technology,the key difference flavor compounds in the upper and lower layers of original strong-flavorBaijiu was screened by partial least squares-discriminant analysis(PLS-DA)model,and the electronic nose results were analyzed by principal component analysis(PCA)and linear discriminant analysis(LDA).The results showed that a total of 76 volatile flavor compounds were identified,including 39 esters,9 acids,9 aromatics,8 alcohols,4 ketones,3 aldehydes,3 furans and 1 other,and the total contents of esters,acids,acetals and organic acids in the lower layers of original strong-flavor Baijiu were significantly higher than those in the upper layers of original strong-flavor Baijiu(P<0.05).Among them,13 volatile flavor compounds such as isoamyl acetate,ethyl acetate,2-hexanone,hexanal,butyric acid,3-methylbutyric acid,and ethyl caproate were the key difference flavor compounds.In addition,electronic nose re-sults could distinguish the upper and lower layers of original strong-flavorBaijiu by PCA and LDA,which could assist HS-SPME-GC-MS to analyze the volatile flavor components in original strong-flavor Baijiu with different spatial levels,and provide a certain theoretical basis for ensuring the quality stability of originalstrong-flavor Baijiu.

electronic noseoriginal strong-flavor Baijiuvolatile flavor compoundkey difference flavor component

李俣珠、杨康卓、刘志鹏、何张兰、郑佳

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宜宾五粮液股份有限公司,四川 宜宾 644000

电子鼻 浓香型原酒 挥发性风味成分 关键差异风味物质

宜宾市高新科技项目

2021GH001

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(1)
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