首页|浓香型白酒在新、老窖池发酵过程中酒醅微生物群落结构差异分析

浓香型白酒在新、老窖池发酵过程中酒醅微生物群落结构差异分析

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为了分析新、老窖池浓香型白酒发酵过程中微生物群落演替特征及差异性,采用高通量测序技术分析酒醅的微生物群落演替与组成结构,并通过相关性网络分析阐明新、老窖池酒醅中微生物群落的相关性与稳定性。结果表明,老窖池酒醅样品中细菌和真菌菌群的多样性均低于新窖池,细菌菌群多样性的增长趋势也要滞后于新窖池,而真菌菌群多样性则相反。新、老窖池酒醅样品中的真菌群落组成及演替规律存在明显差异,而细菌群落较为相似。在老窖池酒醅样品中,曲霉属(Aspergillus)与哈萨克斯坦酵母属(Kazachstania)的相对丰度更高,而在新窖池酒醅样品中酿酒酵母(Saccharomyces)更具优势。另外,相关性网络分析显示,老窖池酒醅的微生物互作网络具有更高的聚集程度,微生物群落的共存关系也更加复杂,稳定性更高。
Differences of microbial community structure in fermented grains during strong-flavor Baijiu fermentationin new and old pits
In order to analyze the succession characteristics and differences of microbial communities of strong-flavor(Nongxiangxing)Baijiu during the fermentation process in the new and old pits,the succession,composition and structure of microbial community in fermented grainswere analyzed by high-throughput sequencing techniques,and the correlation and stability of microbial communities in fermented grains of new and old pits were elucidated by correlation network analysis.The results showed that the diversity of bacteria and fungi in old pits was lower than that in new pits,and the growth trend of bacterial diversity in old pits lagged behind that in new pits,while the diversity of fungi was the opposite.There were obvious dif-ferences in the composition and succession of fungal communities,but the bacterial communities were similar.The relative abundance of Aspergillus and Kazachstania in fermented grains of old pits was higher,but the relative abundance of Saccharomyces was higher in fermented grains of new pits.In addition,the correlation network analysis showed that the microbial network in fermented grains of old pits had a higher degree of aggregation,and the coexistence of microbial communities was more complex and more stable.

strong-flavor Baijiupitsfermented grainsmicrobial diversityhigh-throughput sequencing

邱显平、黄桥、杨静、田蕾、刘英、林宜锦、关统伟

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四川全兴酒业有限公司,四川成都 611637

西华大学食品与生物工程学院,四川成都 610039

食品微生物四川省重点实验室,四川成都 610039

浓香型白酒 窖池 酒醅 微生物多样性 高通量测序

企业重大科技攻关计划

RH2300001318

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(1)
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