Diversity analysis and isolation strains identification of Staphylococcus in high-temperature Daqu
In order to further explore the diversity of Staphylococcus in high-temperature Daqu of different colors in Xiangyang,Hubei Province,the sample sequences with the relative content of Staphylococcus＞10％were downloaded and screened,and the diversity of Staphylococcus in Daqu was analyzed by using Illumina MiSeq high-throughput sequencing technology and combining with traditional pure culture techniques.The results showed that there were 13 samples with a relative content of Staphylococcus＞10％in high-temperature Daqu,including 7 black Daqu,4 yellow Daqu,and 2 white Daqu.The relative abundance of Staphylococcus gene sequence accounted for 14.33％-74.19％of all prokaryotes.Diversity analy-sis showed that the abundance and diversity of Staphylococcus in black Daqu were significantly higher(P＜0.05),and the community structure of Staphylococcus in high-temperature Daqu with different colors showed significant differences(P＜0.05).Operational taxonomic unit(OTU)analysis showed that there were 5 OTU in high-temperature Daqu,with a cumulative average relative content of 91.38％.Traditional pure culture techniques indicated that Staphylococcus saprophyticus was a common cultivable strain in black,yellow,and white Daqu,and was also the main cultivable strain of Staphylococcus in high-temperature Daqu.The results indicated that the abundance and diversity of Staphylococcus were higher in black Daqu,and S.saprophyticus was the main cultivable strain of Staphylococcus in high-temperature Daqu.
high-temperature DaquStaphylococcushigh throughput sequencingoperational taxonomic unittraditional pure culture techniques
NETL
NSTL
万方数据
维普
