摘要
为选育产广谱细菌素乳酸菌,采用琼脂扩散法对甘肃牧区传统发酵乳品中分离纯化的96株乳酸菌进行了筛选,并确定产细菌素菌株,具有最佳抑菌效果的菌株经重离子束12C6+辐照诱变,再采用培养皿分区法进行6种指示菌广谱抑菌性能比较.结果表明,琼脂扩散法筛选出抑菌效果最佳的产细菌素菌株Lp1,经辐照诱变得到98株突变菌,选育出对大肠埃希氏菌(Escherichia coli)、金黄色葡萄球菌(Staphylococcus aureus)、单核细胞增生李斯特氏菌(Listeria monocytogenes)、沙门氏菌(Salmonella)、志贺氏菌(Shigella)和克罗诺杆菌(Cronobacter)抑菌性能均明显优于出发菌株Lp1的5株突变株L088、L087、L063、L049、L010进行广谱抑菌试验,其中菌株L088和L063为广谱抑菌性能较好菌株,突变株L088对上述6种指示菌的抑菌扇环半径比出发菌株Lp1抑菌扇环半径分别提高47.81％、33.28％、22.97％、45.25％、21.39％、40.21％,突变株L063对上述6种指示菌的抑菌扇环半径比出发菌株Lp1抑菌扇环半径分别提高54.27％、29.18％、25.37％、58.03％、17.85％、42.13％.采用培养皿分区法可同时在1个培养皿中完成检测6种指示菌的抑菌试验,可高效筛选产广谱细菌素乳酸菌.
Abstract
To breed broad-spectrum bacteriocin-producing lactic acid bacteria,96 strains of lactic acid bacteria isolated and purified from tradition fer-mented dairy products in Gansu province were screened by agar diffusion assay,and the bacteriocin-producing strains were determined.The strains with the optimal antibacterial effect were mutated by heavy ion beam 12C6+irradiation,and the broad-spectrum antibacterial performance on six indi-cator bacteria was compared by culture dish partition method.The results showed that the bactericin-producing strain Lp 1 with the optimal bacterio-static effect was selected,and 98 mutant strains were obtained by irradiation mutation.Five mutant strains(L088,L087,L063,L049,L010)with significantly better antibacterial performance against Escherichia coli,Staphylococcus aureus,Listeria monocytogenes,Salmonella,Shigella and Cronobacter were screened for broad-spectrum antibacterial performance,and were significantly better than those of the original strain Lpl.Among them,strains L088 and L063 had better broad-spectrum antibacterial performance,the radius of inhibition annular sector of the mutant strain L088 against 6 indicator bacteria increased by 47.81％,33.28％,22.97％,45.25％,21.39％,and 40.21％than that of the original strain Lp1 respectively,and the radius of inhibition annular sector of the mutant strain L063 against 6 indicator bacteria increased 54.27％,29.18％,25.37％,58.03％,17.85％,and 42.13％,respectively.The inhibition tests of 6 indicator bacteria could be completed in one Petri dish simultaneously,which could screen the broad-spectrum bacteriocin-producing lactic acid bacteria efficiently.
基金项目
甘肃省科学院应用研究与开发项目(2020JK-01)
甘肃省科技计划(22YF7WA065)
甘肃省自然科学基金(21JR1RA347)
NETL
NSTL
维普
万方数据