Research on solid-state fermentation conditions of distiller's grains by compound probiotics
Using the distiller's grains(a by-product during Baijiu brewing),mushroom bran,bran and washed rice bran as raw materials,the solid-state fermentation was carried out by adding a high biological activity starter culture prepared with 1∶1∶1(V/V)of Bacillus K,Saccharomyces cerevisiae S and Enterococcus faecium Q.The optimal formula was determined by pH and protein quality before and after fermentation,and the fermentation con-ditions were optimized by single factor and orthogonal tests.The results showed that when distiller's grains,mushroom bran,bran and washed rice bran mass ratio was 1∶5∶2∶2,the pH decreased by 0.22,the contents of crude protein(CP),acid soluble protein(ASP)and ASP/CP increased by 9.94％,66.03％and 51.29％,respectively.The optimum fermentation conditions were determined as follows:fermentation time 3 d,temperature 43 ℃,moisture 50％,inoculum 10.5％.Under these optimal conditions,the contents of CP,ASP,ASP/CP and protein solubility increased by 11.06％,95.77％,76.23％and 14.84％,respectively.The study showed that solid-state fermentation of distillers'grains with compound probiotics could improve the protein quality of distillers'grains and laid a foundation for the secondary utilization of distillers'grains.
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NSTL
万方数据
维普
