Screening and process optimization of strain biotransforming anthocyanidin from pitaya pericarp
Pitaya pericarp powder was fermented with 20 edible fungi as the fermentation substrate.The tested fungus with the highest conversion rate was screened as the fermentation strain of pitaya pericarp by measuring the change of anthocyanin content in fermentation liquid after fermentation,and the fermentation process was optimized by response surface method.The results showed that the content of anthocyanins in pitaya pericarp liquid fermented by Armillaria mellea was the highest among the 20 tested edible fungi.The optimized fermentation conditions were obtained as follows:fermentation time 5 d,inoculum 6%,temperature 25℃,material-liquid ratio 1∶50(g∶ml).Under the optimized conditions,the content of anthocyanidin in the fermentation broth was(2.03±0.02)mg/100 ml,which was 11.94 times that of the blank control without fungal transformation,indicating that microbial assistance could effectively transform anthocyanin in pitaya pericarp.