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火龙果皮花青素生物转化菌株筛选及工艺优化

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该研究以火龙果皮粉为发酵基质,采用20种食用真菌进行发酵,通过监测发酵后发酵液中花青素含量的变化,筛选出转化效率高的供试菌株作为火龙果皮发酵菌株,并采用响应面法优化其发酵工艺。结果表明,20种食用真菌中,经蜜环菌(Armillaria mellea)发酵的火龙果皮液中花青素含量最高,其最优发酵工艺为发酵时间5 d、接种量6%、发酵温度25℃、料液比1∶50(g∶mL)。在此优化条件下,发酵液中花青素含量达(2。03±0。02)mg/100 mL,是未经食用真菌转化的11。94倍,表明微生物辅助可有效转化火龙果果皮中花青素。
Screening and process optimization of strain biotransforming anthocyanidin from pitaya pericarp
Pitaya pericarp powder was fermented with 20 edible fungi as the fermentation substrate.The tested fungus with the highest conversion rate was screened as the fermentation strain of pitaya pericarp by measuring the change of anthocyanin content in fermentation liquid after fermentation,and the fermentation process was optimized by response surface method.The results showed that the content of anthocyanins in pitaya pericarp liquid fermented by Armillaria mellea was the highest among the 20 tested edible fungi.The optimized fermentation conditions were obtained as follows:fermentation time 5 d,inoculum 6%,temperature 25℃,material-liquid ratio 1∶50(g∶ml).Under the optimized conditions,the content of anthocyanidin in the fermentation broth was(2.03±0.02)mg/100 ml,which was 11.94 times that of the blank control without fungal transformation,indicating that microbial assistance could effectively transform anthocyanin in pitaya pericarp.

pitaya pericarpanthocyanidinbiotransformationedible fungusscreeningprocess optimization

程水明、李露欣、刘杰凤

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广东石油化工学院生物工程系,广东茂名 525000

火龙果果皮 花青素 生物转化 食用真菌 筛选 工艺优化

广东省科技专项目

mmkj2020048

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(1)
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