UPLC-MS/MS法同时测定白酒中21种氨基酸的含量
Simultaneous determination of 21 amino acids in Baijiu by UPLC-MS/MS method
张季 1杨沙 1罗玉航 1侯睿 1马新雨 2朱小兰3
作者信息
- 1. 遵义市产品质量检验检测院,贵州遵义 563099
- 2. 中国农业大学 四川现代农业产业研究院,四川成都 611430
- 3. 安捷伦科技(中国)有限公司成都分公司,四川成都 610213
- 折叠
摘要
该研究建立了一种采用超高效液相色谱-串联质谱(UPLC-MS/MS)同时测定白酒中21种氨基酸含量的检测方法.白酒样品经过12 000 r/min离心5 min后,将上清液直接用于色谱分析,外标法定量.结果表明,在10～1 000 ng/mL质量浓度范围内,氨基酸含量线性关系良好,相关系数(R2)均＞0.995,检出限和定量限分别为0.1～5.0 ng/mL和0.2～20 ng/mL,其保留时间相对标准偏差(RSD)＜0.29％,峰面积RSD为1.54％～10.33％,加标回收率为80％～130％.该方法准确可靠,重复性好,可以用于白酒中21种氨基酸的含量的测定.
Abstract
A method was developed for the simultaneous determination of 21 amino acids in Baijiu using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).After 12 000 r/min centrifugation of Baijiu samples for 5 min,the supernatant was filtered through a mem-brane for instrumental analysis and quantified by external standard method.The results showed that the standard curve was linear over the mass con-centration range of 10-1 000 ng/ml with the correlation coefficient(R2)greater than 0.995,the limits of detection were 0.1-5.0 ng/ml and the limits of quantification were 0.2-20 ng/ml,the relative standard deviation(RSD)of the retention time was less than 0.29％,the RSD of the peak area repeatability was in the range of 1.54％-10.33％,and the recovery rate of the target substance was 80％-130％.The method was accurate,reliable and reproducible,and could be used for the determination of 21 amino acids in Baijiu.
关键词
白酒/氨基酸/超高效液相色谱-串联质谱法Key words
Baijiu/amino acid/ultra performance liquid chromatography-mass spectrometry引用本文复制引用
基金项目
贵州省科技计划(黔科合支撑[2023]般386)
贵州省科技计划(黔科合支撑[2023]一般364)
国家市场监督管理总局科技项目(2022MK117)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据