Simultaneous determination of 21 amino acids in Baijiu by UPLC-MS/MS method
A method was developed for the simultaneous determination of 21 amino acids in Baijiu using ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).After 12 000 r/min centrifugation of Baijiu samples for 5 min,the supernatant was filtered through a mem-brane for instrumental analysis and quantified by external standard method.The results showed that the standard curve was linear over the mass con-centration range of 10-1 000 ng/ml with the correlation coefficient(R2)greater than 0.995,the limits of detection were 0.1-5.0 ng/ml and the limits of quantification were 0.2-20 ng/ml,the relative standard deviation(RSD)of the retention time was less than 0.29％,the RSD of the peak area repeatability was in the range of 1.54％-10.33％,and the recovery rate of the target substance was 80％-130％.The method was accurate,reliable and reproducible,and could be used for the determination of 21 amino acids in Baijiu.
NETL
NSTL
万方数据
维普
