Isolation,identification and biological characteristics of high-yield amylase and protease bacteria from high-temperature Daqu
Strains were isolated from high-temperature Daqu by dilution plate method and plate scribing method,and strains with high amylase and protease production were screened by transparent circle method and enzyme activity determination.Strains were identified by morphological observa-tion and molecular biology techniques,and their resistance to high temperature and acid was studied.The results showed that a strain with high-yield amylase and protease was screened and numbered as KC-6,and the amylase and protease activities were 64.45 U/ml and 0.60 U/ml,respectively.The strain KC-6 was identified as Bacillus thuringiensis,which could withstand high temperature of 60 ℃.It grew slowly in strong acidic conditions and rapidly in weak acid and neutral environments.
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维普
