首页|酿酒专用小麦大曲中挥发性风味成分与微生物群落相关性分析

酿酒专用小麦大曲中挥发性风味成分与微生物群落相关性分析

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为研究制曲原料对大曲中挥发性风味成分和微生物群落的影响,以酿酒专用小麦大曲为研究对象,普通小麦大曲为对照,分别采用顶空固相微萃取-气质联用法和高通量测序技术分析大曲的挥发性风味成分和微生物群落组成,并基于多元统计分析方法探讨二者相关性。结果表明,两种小麦大曲共检出125种挥发性风味成分,共筛选出49种差异风味成分,酿酒专用小麦大曲中差异挥发性风味成分种类及含量优于普通小麦大曲。在微生物群落组成上共检出优势细菌属11种、优势真菌属4种;相关性分析表明,乳杆菌属(Lactobacillus)和曲霉菌属(Aspergillus)与醇类含量呈显著正相关(P<0。05),芽孢杆菌属(Bacillus)与吡嗪类含量呈高度显著正相关(P<0。001),泛菌属(Pantoea)、明串珠菌属(Leuconostoc)和葡萄球菌属(Staphylococcus)是醛类主要贡献微生物。采用酿酒专用小麦制曲有益于大曲中细菌的富集培养及挥发性风味成分的丰富和均衡。
Correlation analysis of volatile flavor components and microbial community in Daqu made by special wheat for brewing
In order to study the effects of raw materials on volatile flavor components and microbial communities in Daqu,using Daqu made by special wheat for brewing as the research object and Daqu made by common wheat as the control,the volatile flavor components and microbial community composition of Daqu were analyzed by using headspace solid-phase microextraction coupled with gas chromatography-mass spectrometry and high-throughput sequencing technology respectively,and their relationships were explored based on multivariate statistical analysis.The results showed that a total of 125 volatile flavor components were detected in the two kinds of wheat Daqu,and 49 difference flavor components were screened out,and the types and contents of difference volatile flavor components in the special wheat Daqu were better than those in the common wheat Daqu.In the composition of microbial communities,11 species of dominant bacterial genera and 4 species of dominant fungal genera were detected.Correla-tion analysis showed that Lactobacillus and Aspergillus were significantly positive correlated with the content of alcohols(P<0.05),Bacillus was highly significantly positive correlated with the content of pyrazines(P<0.001),and Pantoea,Leuconostoc and Staphylococcus were the main contributing mi-croorganisms of aldehydes.The use of the special wheat to produce Daqu was beneficial to the enrichment culture of bacteria and the enrichment and balance of volatile flavor components in Daqu.

wheat for brewingvolatile flavor componentmicrobial communityprincipal component analysiscorrelation

王洋、谢菲、杜礼泉、范昌明、冯波

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四川省绵阳市丰谷酒业有限责任公司,四川绵阳 621000

酿酒专用小麦 挥发性风味成分 微生物群落 主成分分析 相关性

四川省重点研发项目

2022YFS0550

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(2)
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