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泸州产区浓香型白酒酿造微环境-风味相关性分析

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该研究分别采用高通量测序技术结合顶空固相微萃取-全二维气质联用(HS-SPME-GCxGC-MS)技术对泸州产区浓香型白酒厂来源大曲、糟醅和窖泥等酿造微环境的微生物菌群结构及大曲、糟醅、窖泥及原酒中挥发性风味物质进行分析,并对其相关性进行研究。结果表明,土壤样本微生物群落丰富度和多样性均高于大曲、糟醅和窖泥样本。大曲、糟醅、窖泥和原酒中共检测出182种挥发性风味化合物,包括68种酯类、27种醇类、23种酸类和64种其他化合物,不同样本中风味化合物含量差异明显。其中,酯类、醇类、酸类化合物分别在窖泥、大曲、糟醅中含量最高,分别达到78。63%、43。57%和24。65%。相关性分析结果表明,酿造微环境理化性质、微生物群落与挥发性风味化合物的形成相关,且细菌菌群与挥发性风味化合物的相关性强于真菌菌群。其中,白腐菌属(Petrimonas)、喜热菌属(Caloramator)等对泸州产区浓香型白酒中酯类化合物的形成有重要贡献,而醋酸乳杆菌属(Acetilactobacillus)、乳杆菌属(Lactobacillus)等对醇类和酸类化合物的形成有重要贡献。
Correlation analysis between brewing microenvironment and flavor of strong-flavor Baijiu in Luzhou producing area
The microbial community structure of microorganisms in microenvironment such as Daqu,fermented grains and pit mud of strong-flavor(Nongxiangxing)Baijiu distillery in Luzhou producing area and volatile flavor compounds in Daqu,fermented grains,pit mud and base liquor were analyzed by high-throughput sequencing technology combined with headspace solid-phase microextraction-comprehensive two-dimensional gas chromatography-mass spectrometry(HS-SPME-GCxGC-MS)respectively,and their correlation was studied.The results showed that the abun-dance and diversity of microbial communities in soil samples were higher than those in Daqu,Zaopei,and pit mud samples.A total of 182 volatile flavor compounds were detected in Daqu,Zaopei,pit mud and base liquor,including 68 esters,27 alcohols,23 acids and 64 other compounds.There were significant differences in the content of flavor compounds among different samples.Among them,the esters,alcohols and acids had the highest content in pit mud,Daqu and Zaopei,which were 78.63%,43.57%and 24.65%,respectively.The results of the correlation analysis indicated that the physicochemical properties,microbial communities of the brewing microenvironment,and the formation of volatile flavor compounds were correlated.Furthermore,the correlation between bacterial communities and volatile flavor compounds was stronger than that of fungal communities.Among them,the Petrimonas,Caloramator and other genera played important roles in the formation of ester compounds of strong-flavor Baijiu in Luzhou producing area.On the other hand,the Acetilactobacillus,Lactobacillus and other genera played crucial roles in the formation of alcohol and acid compounds.

Luzhou producing areastrong-flavor Baijiubrewing microenvironmentmicrobial community structurevolatile flavor compounds

贺雨杰、唐雨润、蒋小清、郭晴艳

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西华大学 食品与生物工程学院,四川成都 610039

西华大学食品微生物四川省重点实验室,四川成都 610039

泸州产区 浓香型白酒 酿造微环境 微生物群落结构 挥发性风味物质

大学生创新创业训练计划项目西华大学人才引进项目

S202210623038Z211045

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(2)
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