Using feruloyl esterase activity as the response value,the conditions for producing feruloyl esterase by Aspergillus awamori were optimized through single factor tests and response surface experiments.The results showed that the optimal fermentation conditions for feruloyl esterase production by A.awamori were as follows:fermentation time 4 d,glucose addition 14.28 g/L,yeast extract powder addition 3.88 g/L,inoculum 9.70％.Under the optimized conditions,the feruloyl esterase activity reached(3 912.32±34.34)mU/ml,which was 1.89 times that of before optimization.
关键词
泡盛曲霉/阿魏酸酯酶/响应面法/产酶条件优化
Key words
Aspergillus awamori/feruloyl esterase/response surface methodology/optimization of enzyme production conditions
维普
万方数据