In order to improve the comprehensive production performance of medium-high-temperature Daqu in strong-flavor(nongxiangxing)Baijiu,strains were separated and screened from medium-high-temperature Daqu,and 2,3,5-triphenyltetrazolium chloride(TCC)was used for preliminary screening,and the fermentation experiments were used for rescreening to obtain yeast with good fermentation performance.The Z comprehensive score was used as the response value.Through single factor and response surface test,the inoculation of screened strains was optimized,and it was used to strengthen Daqu production.The results showed that a total of 8 strains were isolated and purified,among which 3 strains of yeast Y1,Y2,and Y3 with good alcohol production performance were screened out.The inoculum of 3 strains of yeast was 7.0×108 CFU/ml,3.0×108 CFU/ml,1.5×108 CFU/ml.Under the optimized combination conditions,the overall score of principal component Z was 513.The fermentation power,saccharification power and liquefaction power were 3.46 g/(g·72 h),994 mg/(g·h)and 1.12 g/(g·h),respectively,which were increased by 424％,6.7％and 5.7％compared with before optimization.
维普
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