Isolation,screening and enzyme-producing characteristics of Bacillus from Yimen Douchi
In this study,the Bacillus producing amylase,protease,lipase and cellulase were isolated and screened from Yimen Douchi,a traditional fermented soybean product in Yunnan Province.The enzyme-producing strains were screened by hydrolyzing ring method and shaking flask fermen-tation.The growth performance analysis and molecular biological identification of the strains with high enzyme production were carried out.The results showed that 64 strains were isolated and purified from Yimen Douchi,and one strain of NB-13 with good ability to produce amylase and protease was screened,its amylase and protease activities were 100.64 U/ml and 34.30 U/ml,respectively.One strain ND-21 had good lipase production ability,and the lipase activity was 39.95 U/ml.Two strains with good cellulase-producing ability,strains ND-38 and NB-55 had cellulase activities of 1.65 U/ml and 1.72 U/ml,respectively.According to the results of growth situation,strains ND-21 and NB-13 had the most enriched bacteria and the strongest growth ability.The strains NB-13 and NB-55 were identified as Bacillus subtilis and Bacillus amyloliquefaciens,respectively,and strains ND-21,ND-38 were identified as Bacillus licheniformis.
DouchiBacillusisolation and screeningidentificationenzyme productiongrowth performance
万方数据
维普
