Antibacterial activity of glucose oxidase and Lactobacillus plantarum
The antibacterial effects of glucose oxidase(GOD)and Lactobacillus plantarum on Escherichia coli and Salmonella from chickens and pigs were determined by Oxford cup method.The results showed that GOD had antibacterial activity against pathogenic bacteria from different sources detected by the Oxford Cup method,and its antibacterial activity increased with increasing concentration within a certain concentration range.It still had antibacterial activity at enzyme concentration of 10 U/ml,and the diameter of the antibacterial zone were all ≥ 9.25 mm.GOD enzyme still had good antibacterial activity after 10 min dry heat treatment at 37-75 ℃.The strongest antibacterial activity was observed against E.coli K88 from pig at 65 ℃ and Salmonella 0533 at 37 ℃,with inhibitory zone diameters of 10.75 mm and 12.25 mm,respectively.The diameter of the inhibitory zone of L.plantarum BLCC2-0126 against the tested pathogenic bacteria was the largest for tested pathogens,while the diameter of the inhibitory zone against E.coli K99 and Salmonella 0533 from pig was 13.75 mm.In summary,both GOD and BLCC2-0126 had antibacterial effects and could be used to-gether in practical production.
glucose oxidaseLactobacillus plantarumOxford cup methodantibacterial effect