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枸杞茶醋醋酸发酵工艺优化及抗氧化活性研究

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该研究以枸杞芽茶和玉米须为原料,采用巴氏醋杆菌(Acetobacter pasteurianus)AC-2005发酵制备枸杞茶醋,以总酸含量为评价指标,通过单因素试验及正交试验优化枸杞茶醋醋酸发酵工艺,对其理化指标进行测定,并对其发酵前后的功能活性成分和抗氧化活性变化进行分析。结果表明,枸杞茶醋的最优醋酸发酵工艺为初始酒精度9%vol,巴氏醋杆菌AC-2005接种量10%,发酵温度30 ℃,初始pH值3。5。在此优化条件下,枸杞茶醋的总酸含量为7。10g/100 mL,pH值为3。56,还原糖含量为1。70g/100mL,可溶性固形物含量为9。28 g/100 mL,其理化指标符合相关标准要求。与发酵前相比,枸杞茶醋中的总酚(2。27 mg/mL)和总黄酮含量(0。94 mg/mL)显著升高(P<0。05),甜菜碱含量(2。30 mg/mL)无显著变化(P>0。05),类胡萝卜素含量(0。62 mg/mL)显著降低(P<0。05);抗氧化能力显著升高(P<0。05),1,1-二苯基-2-三硝基苯肼(DPPH)、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)自由基清除率和铁离子还原/抗氧化能力(FRAP)分别为20。27%、12。71%和11。68%。
Optimization of acetic acid fermentation process and antioxidant activity of Lycium barbarum tea vinegar
In this study,Lycium barbarum tea vinegar was prepared by Acetobacter pasteurianus AC-2005 fermentation using L.barbarum bud tea and corn whisker as raw materials.Using total acid content as evaluation index,the acetic acid fermentation process of L.barbarum tea vinegar was opti-mized by single factor and orthogonal tests.The physicochemical indexes were determined,and the changes in functional active components and an-tioxidant activity before and after fermentation were analyzed.The results showed that the optimal acetic acid fermentation process of L.barbarum tea vinegar was initial alcohol content 9%vol,A.pasteurianus AC-2005 inoculum 10%,fermentation temperature 30 ℃,and initial pH 3.5.Under the conditions,the total acid content,pH,reducing sugar and soluble solids contents of L.barbarum tea vinegar were 7.10 g/100 ml,3.56,1.70 g/100 ml and 9.28 g/100 ml,respectively,and its physical and chemical indicators met the requirements of the relevant standards.Compared with that before fermentation,the total phenol(2.27 mg/ml)and total flavonoid content(0.94 mg/ml)in L.barbarum tea vinegar were significantly higher(P<0.05),the betaine content(2.30 mg/ml)did not change significantly(P>0.05),the carotenoid content(0.62 mg/ml)was significantly decreased(P<0.05),the antioxidant capacity was significantly higher(P<0.05),and the 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azinobis-(3-ethylbenzthiazoline-6-sulphonate)(ABTS)and ferric ion reducing antioxidant power(FRAP)were 20.27%,12.71%and 11.68%,respectively.

Lycium barbarum tea vinegaracetic acid fermentationprocess optimizationfunctional active componentsantioxidant activity

强晓、张紫瑗、耿贝贝、李天萌、袁宇轩、夏婷

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天津科技大学生物工程学院食品营养与安全国家重点实验室天津市微生物代谢与发酵过程控制技术工程中心,天津 300457

枸杞茶醋 醋酸发酵 工艺优化 功能活性成分 抗氧化活性

宁夏回族自治区重点研发计划天津市研究生科研创新项目

2022BBF020102022SKYZ067

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(3)
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