Optimization of extraction conditions for chlorogenic acid extraction from honeysuckle waste residue and antioxidant activity
To study the extraction rate of chlorogenic acid from honeysuckle waste residue and its antioxidant activity changes after fermentation,hon-eysuckle waste residue was fermented by Bacillus subtilis and Aspergillus niger(1∶1),the extraction conditions were optimized using single factor and response surface tests,and the changes in antioxidant activity of honeysuckle extract before and after fermentation were investigated.The results showed that the optimal extraction conditions were fermentation time 71 h,temperature 32 ℃,glucose addition 3％and inoculum 1.5％.Under these optimal conditions,the chlorogenic acid extraction rate of the fermented group of honeysuckle waste increased from 1.12％to 1.63％compared with that of the unfermented group.Compared with the unfermented group,the DPPH radical scavenging rate of honeysuckle extract in the fermentation group increased from 82.96％to 86.64％,the ABTS free radical scavenging rate increased from 82.17％to 86.34％,and the Fe3+reducing power in-creased from 1.03 to 1.09.The results indicated that the mixed fermentation of B.subtilis and A.niger could effectively increase the chlorogenic acid extraction rate of the honeysuckle waste residue,and could enhance the antioxidant activity of honeysuckle waste residue.
万方数据
维普
