紫外线诱变选育高产豆豉纤溶酶菌株及其产酶条件优化
Breeding of high-yield Douchi fibrinolytic enzyme strain by ultraviolet mutagenesis and optimization of enzyme-producing conditions
张文勇 1张恒慧 1王晓丽1
作者信息
- 1. 太原工业学院环境与安全工程系,山西太原 030008
- 折叠
摘要
该研究以分离自豆豉样品中产豆豉纤溶酶的枯草芽孢杆菌(Bacillus subtilis)DC-1为出发菌株,采用紫外线诱变选育高产豆豉纤溶酶且稳定遗传的菌株,并以豆豉纤溶酶活力为评价指标,通过单因素试验及正交试验对其发酵条件进行优化.结果表明,筛选出一株高产豆豉纤溶酶活力且稳定遗传的诱变菌株DC-V5,其最优发酵条件为:接种量3％、装液量70 mL/250 mL、发酵温度34℃、初始pH 6.5.在此优化发酵条件下,诱变菌株DC-V5产豆豉纤溶酶活力最高达到(451.26±11.09)IU/mL,是优化前出发菌株DC-1的1.74倍.
Abstract
Using Douchi fibrinolytic enzyme-producing strain Bacillus subtilis DC-1 isolated from Douchi samples as the original strain,the high-yield Douchi fibrinolytic enzyme strain with stable inheritance was bred by ultraviolet mutagenesis,and the fermentation conditions were optimized by sin-gle factor tests and orthogonal tests using Douchi fibrinolytic enzyme activity as evaluation index.The results showed that a mutagenic strain DC-V5 with high Douchi fibrinolytic enzyme activity and genetic stability was obtained,and the optimal fermentation conditions were as follows:inoculum 3％,liquid loading volume 70 ml/250 ml,fermentation temperature 34 ℃ and initial pH 6.5.Under the optimal fermentation conditions,the Douchi fibrinolytic enzyme activity of strain DC-V5 reached(451.26±11.09)IU/ml,which was 1.74 times that of the original strain DC-1.
关键词
枯草芽孢杆菌/豆豉纤溶酶/紫外线诱变/产酶条件优化Key words
Bacillus subtilis/Douchi fibrinolytic enzyme/ultraviolet mutagenesis/enzyme-producing condition optimization引用本文复制引用
基金项目
大学生创新项目(S202314101143)
大学生创新项目(GK2023096)
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据