Optimization of medium compositions of Retiboletus sinensis and its influence on mycorrhizal synthesis and endogenous hormones of Pinus thunbergii
In this study,using the mycelial dry mass of Retiboletus sinensis as the response value,the liquid fermentation medium of R.sinensis was optimized by using Plackett-Burman test,steepest climb test and response surface test,and its effect on the content of plant endogenous hormones of the associated Pinus thunbergii was investigated.The results showed that the optimal culture medium compositions for R.sinensis was:KH2PO4 0.5 g/L,MgSO4·7H2O 0.5 g/L,ZnSO4 3 g/L,ammonium tartrate 0.75 g/L,ferric citrate 10 g/L,thiamine 50 μg/L,casein hydrolysate 0.99 g/L,yeast extract 1.35 g/L,and glucose 18.2 g/L.Under these conditions,the mycelial dry mass of R.sinensis was the highest,reaching 3.43 g/L.After P.thunbergii was inoculated with R.sinensis and formed mycorrhiza,the root mycorrhizal seedlings had a two-branched structure and a clear harpin network under a microscope.The contents of plant hormones in P.thunbergii mycorrhizal seedlings such as indole-3-acetic acid(IAA),zeatin acid(ZT)and gibberellin acid(GA)increased by 33.0％,42.5％and 102.0％,respectively,while the content of abscisic acid(ABA)decreased by 29.0％.
万方数据
维普
