Sterilization effect of cold plasma sterilization technology on the mixed microbial system of Paocai-derived microorganism
The mixed microbial system composed of 3 kinds of lactic acid bacteria and 2 kinds of yeasts from Paocai was prepared.The sterilization effect of different cold plasma sterilization(CPS)treatments on Paocai-derived microorganism simulated mixed microbial system were investigated,and the film production,gas production,acid production,and acid resistance of the mixed microbial system were evaluated.The results showed that when the discharge voltage and treatment time were the same,the survival rates of the 5 microorganisms decreased with the increase of the treatment time and discharge voltage.When the treatment time was greater than 9 min,the discharge voltage was higher,the membrane accumulation and the gas production capacity decreased,but the difference of gas production effect was not obvious.The optimal CPS treatment conditions were discharge voltage 160 kV and treatment time 9 min.Under the optimized conditions,the microbial biomass(OD600nm value)in the mixed microbial system was the lowest(1.35)when fermentation for 31 h,which effectively slowed down the growth of lactic acid bacteria and yeasts.The pH was the highest(4.12),which inhibited acid production by microorganism and alleviated post-acidification to some extent.
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