摘要
该研究建立了高效液相色谱法(HPLC)同时测定人参保健酒中11种人参皂苷(人参皂苷Rg1、Re、Rf、Ra2、Rb1、Rc、Ra1、Ro、Rb2、Rb3、Rd)的方法.采用Agilent SB C18色谱柱(4.6 mm×250 mm,5 μm),以乙腈-0.1％磷酸为流动相梯度洗脱,流速1.0 mL/min,柱温28 ℃,人参皂苷的检测波长203 nm,进样量10 μL的条件进行测定.结果表明,11种人参皂苷在各自浓度范围内线性关系良好(R2≥0.999 9),检出限在1.52～3.03 mg/L,定量限在4.59～9.18 mg/L,该方法精密度试验、稳定性试验、重复性试验结果相对标准偏差(RSD)均小于5％,平均加标回收率95.39％～101.96％,RSD为0.07％～2.46％.该方法操作简单,准确度高,重复性好,可用于人参保健酒中11种人参皂苷含量测定.
Abstract
In this study,a high performance liquid chromatography(HPLC)method was established for the simultaneous determination of 11 ginsenosides(ginsenoside Rg1,Re,Rf,Ra2,Rb1,Rc,Ra1,Ro,Rb2,Rb3,Rd)in ginseng health wine.Using Agilent SB C18 column(4.6 mm×250 mm,5 μm),with acetonitrile-0.1％phosphoric acid as mobile phase gradient elution,flow rate 1.0 ml/min,column temperature 28 ℃,the detection wavelength of gin-senoside was 203 nm,and the injection volume was 10 μl.The results showed that 11 ginsenosides all had good linear relationship in their respective concentration range(R2≥0.999 9).The limits of detection were 1.52-3.03 mg/L,and the limits of quantitation were 4.59-9.18 mg/L.The relative standard deviation(RSD)of precision test,stability test and repetition test was less than 5％.The average recovery rate was 95.39％-101.96％,RSD was 0.07％-2.46％.The method had the advantages of simple operation,high accuracy and good repeatability,and could be used for the determination of 11 ginsenosides in ginseng health wine.
维普
万方数据