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微生物合成4-羟基-2,5-二甲基-3(2H)-呋喃酮的研究进展

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4-羟基-2,5-二甲基-3(2H)-呋喃酮(HDMF)是一种具有焦糖味的安全优良的甜味香料和增香剂,其阈值低、香势好且特征性强,被广泛应用于食品、烟草等产品中,市场需求量大,尤其是天然来源的HDMF受到人们更多的青睐.然而,天然来源的HDMF产量较低,尚不能满足当前市场的需求.该文从其应用、检测、生产方法、合成HDMF微生物的种类、微生物合成HDMF的机制与关键酶及其发酵培养影响因素进行简述,重点侧重于已报道的具有合成HDMF的微生物种类、微生物合成HDMF的内在机制及其影响其发酵的培养因素,旨在为后续筛选、改造获得优良高产HDMF微生物菌株,并优化微生物发酵条件实现生物法高效制备天然HDMF提供参考.
Research progress on the synthesis of 4-hydroxy-2,5-dimethyl-3(2H)-furanone by microorganisms
4-hydroxy-2,5-dimethyl-3(2H)-furanone(HDMF)is a safe and excellent sweet flavor and aroma agent with caramel taste,which has a lower threshold,good fragrance potential,and strong characteristics,which is widely used in food,tobacco,and other products,and the market demand is large,especially for natural sources of HDMF.However,the yield of natural HDMF is low and cannot meet the current market demand.For this rea-son,the application,detection,production methods,species of microorganisms that synthesize HDMF,mechanism of microbial synthesis of HDMF,and key enzymes as well as influencing factors of fermentation culture conditions were briefly reviewed.The main focus was on the microorganisms species that have been reported to synthesize HDMF,the internal mechanism of microbial synthesis of HDMF and the culture factors affecting its fer-mentation,aiming to provide a reference for subsequent screening,modification and obtaining excellent and high-yield HDMF strains,and optimize the microbial fermentation conditions to achieve efficient preparation of natural HDMF by biological methods.

4-hydroxy-2,5-dimethyl-3(2H)-furanonemicrobial synthesissynthesis mechanismculture condition

宋金鹏、仲秀芳、李学思、曹振华、彭新辉、范光森

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德州市纤维检验所,山东德州 253000

北京工商大学食品与健康学院,北京 100048

河南牧业经济学院食品与生物工程学院,河南郑州 450046

河南省宋河酒业股份有限公司,河南鹿邑 477265

湖南中烟工业有限责任公司技术中心,湖南长沙 410000

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4-羟基-2,5-二甲基-3(2H)-呋喃酮 微生物合成 合成机制 培养条件

湖南中烟工业有限责任公司科技项目

KY2019JC0032

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(4)
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