Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophyllene
In order to screen the high-yield β-caryophyllene(BCP)strains,the aroma components in light-flavor(Qingxiangxing)Baijiu and Daqu was detected by headspace solid-phase microextraction(HS-SPME)-GC-MS.The β-caryophyllene-producing strains were preliminarily screened by tradi-tional isolation techniques,Fuqu was inoculated with primary screening strains to re-screening by β-caryophyllene content determination,and identified by molecular biology technique.The extraction conditions of β-caryophyllene in Fuqu were optimized,and the screened strains were applied in small tank fermentation.The results showed that the β-caryophyllene content in the light-flavor original liquor was 0.02 g/L,and the relative content ofβ-caryophyllene in Daqu was 0.85％.Five strains of mold(SDA1-SDA5)were preliminarily screened,and the strain SDA3 with the highest relative content of β-caryophyllene(1.04％)was identified as Rhizopus delemar.The β-caryophyllene content was 37.98 mg/L when the extraction reagent was anhydrous ethanol and the extraction time was 2 h.Compared with the addition of Daqu alone,the β-caryophyllene content in liquor produced by adding strain SDA3 to enhance Fuqu for small tank fermentation was increased from 13.65 mg/L to 24.65 mg/L.
β-caryophylleneDaquBaijiumoldscreeningidentificationfortified Fuqu
万方数据
维普
