In order to understand the antibacterial mechanism of bacteriocin from Lactobacillus plantarum YP36,the differential expression proteins of L.plantarum SL32-2 before and after bacteriocin treatment were analyzed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)combined with label-free quantitative proteomics technique,and the metabolic function of the differential proteins were analyzed.The results showed that 1 999 and 2 005 proteins were identified in the fermentation broth of L.plantarum SL32-2 before and after bacteriocin treat-ment respectively,15 and 59 proteins were significantly up-regulated and down-regulated,26 proteins were disappeared,and 33 proteins were in-duced to expression.Among the identified proteins,enzymes accounted for the largest proportion,followed by transporters.Gene Ontology(GO)en-richment analysis showed that there were 99 functional items of significantly differentially expressed proteins,which mainly focused on peptidoglycan catabolism,cell wall macromolecular catabolism,carbohydrate metabolism,pyrimidine nucleotide metabolism and biosynthesis.The Kyoto Encyclo-pedia of Genes and Genomes(KEGG)enrichment analysis showed that the differential proteins involved 53 pathways including starch and sucrose metabolism,alanine,aspartate and glutamate metabolism,cofactor biosynthesis and pyrimidine metabolism.
关键词
细菌素/高效液相色谱-串联质谱/蛋白质组学/抑菌机制/代谢功能分析
Key words
bacteriocin/high performance liquid chromatography tandem mass spectrometry/proteomics/bacteriostatic mechanism/metabolic func-tion analysis
维普
万方数据