Antibacterial mechanism of bacteriocin from Lactobacillus plantarum YP36 based on proteomics technology
In order to understand the antibacterial mechanism of bacteriocin from Lactobacillus plantarum YP36,the differential expression proteins of L.plantarum SL32-2 before and after bacteriocin treatment were analyzed by high performance liquid chromatography-tandem mass spectrometry(HPLC-MS/MS)combined with label-free quantitative proteomics technique,and the metabolic function of the differential proteins were analyzed.The results showed that 1 999 and 2 005 proteins were identified in the fermentation broth of L.plantarum SL32-2 before and after bacteriocin treat-ment respectively,15 and 59 proteins were significantly up-regulated and down-regulated,26 proteins were disappeared,and 33 proteins were in-duced to expression.Among the identified proteins,enzymes accounted for the largest proportion,followed by transporters.Gene Ontology(GO)en-richment analysis showed that there were 99 functional items of significantly differentially expressed proteins,which mainly focused on peptidoglycan catabolism,cell wall macromolecular catabolism,carbohydrate metabolism,pyrimidine nucleotide metabolism and biosynthesis.The Kyoto Encyclo-pedia of Genes and Genomes(KEGG)enrichment analysis showed that the differential proteins involved 53 pathways including starch and sucrose metabolism,alanine,aspartate and glutamate metabolism,cofactor biosynthesis and pyrimidine metabolism.
bacteriocinhigh performance liquid chromatography tandem mass spectrometryproteomicsbacteriostatic mechanismmetabolic func-tion analysis
万方数据
维普
