Knockout and transcriptome sequencing analysis of cdc50 gene in Saccharomyces cerevisiae cells
In order to explore the effect of cdc50 gene on the function of Saccharomyces cerevisiae cells,using PYM 14 plasmid as the template,the cdc50 gene in wild type S.cerevicae BY4742 was knocked out by lithium acetate conversion method,and the growth of mutant strain Δcdc50 was measured.Transcriptomic analysis was performed on wild type yeast strain BY4742 and mutant strain Δcdc50 respectively,differential expression genes(DEGs)of them were screened,and the gene ontology(GO)and the Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis were carried out.The results showed that the S.cerevisiae cdc50 gene deletion strain Δcdc50 was successfully constructed.After gene cdc50 deletion,the yeast cells morphology changed from round to rod,the relative growth rate of strain Δcdc50 was 1.98％after culture for 12 h,which was signifi-cantly lower than that of wild type yeast BY4742(P＜0.05).Compared with wild type yeast BY4742,there were 581 DEGs in strain Δcdc50,among them,271 genes were up-regulated and 310 genes were down-regulated.GO enrichment analysis showed that DEGs mainly concentrated in the oxi-dation-reduction process in biological processes,the oxidoreductase activity,cofactor binding and transmembrane transporter activity in molecular functions.The KEGG enrichment analysis showed that DEGs was mainly concentrated in glycolysis/gluconeogenesis,fatty acid degradation and car-bon metabolism pathways.
万方数据
维普
