中国酿造2024,Vol.43Issue(4) :135-143.DOI:10.11882/j.issn.0254-5071.2024.04.021

新型生物反应器对浓香型酒醅微生物群落及理化指标的影响

Effects of new bioreactor on microbial community and physicochemical indexes of strong-flavor fermented grains

莫玉婷 袁思棋 周帅 税梁杨 杨明永 刘君 杨周林 范宏筠
中国酿造2024,Vol.43Issue(4) :135-143.DOI:10.11882/j.issn.0254-5071.2024.04.021

新型生物反应器对浓香型酒醅微生物群落及理化指标的影响

Effects of new bioreactor on microbial community and physicochemical indexes of strong-flavor fermented grains

莫玉婷 1袁思棋 2周帅 3税梁杨 3杨明永 3刘君 4杨周林 5范宏筠1
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作者信息

  • 1. 四川轻化工大学 生物工程学院,四川 宜宾 644000;泸州国之荣耀酒业有限公司,四川 泸州 646000
  • 2. 四川轻化工大学 生物工程学院,四川 宜宾 644000;泸州老窖集团有限责任公司,四川 泸州 646000;酿酒生物技术及应用四川省重点实验室,四川 宜宾 644000
  • 3. 泸州国之荣耀酒业有限公司,四川 泸州 646000
  • 4. 四川轻化工大学 生物工程学院,四川 宜宾 644000;酿酒生物技术及应用四川省重点实验室,四川 宜宾 644000
  • 5. 四川轻化工大学 生物工程学院,四川 宜宾 644000
  • 折叠

摘要

为生产高品质浓香型白酒,以新型生物反应容器和传统窖池为研究对象,探索传统窖池生产工艺(对照组)、新型生物反应器生产工艺(实验组I)及优化新型生物反应器生产工艺(实验组Ⅱ)对发酵酒醅理化指标、微生物群落的影响,并通过冗余分析(RDA)探讨酒醅发酵过程中理化指标与微生物群落结构的相关性.结果表明,与对照组相比,实验组Ⅰ、实验组Ⅱ升温更快,顶点温度高于对照组(30.0℃),分别为33.2℃、35.5℃,实验组Ⅱ升温效果最优且保持适宜温度时间更长,实验组水分含量、淀粉及还原糖消耗量更高,实验组Ⅱ下层酒醅酸度最高(4.24 mmol/10 g).实验组Ⅱ酒醅在发酵后优势细菌门厚壁菌门(Firmicutes)与优势真菌门为子囊菌门(Ascomycota),相对丰度分别为92.44%、97.59%;优势细菌属乳杆菌属(Lactobacillus)相对丰度最高(92.30%).RDA结果发现,实验组Ⅱ酒醅理化指标与微生物群落结构变化存在显著相关性(P<0.05).

Abstract

In order to produce high quality strong-flavor(Nongxiangxing)Baijiu,using new bioreactor and traditional pit as research objects,the effects of traditional pit production technology(control group),new bioreactor production technology(experimental group Ⅰ)and optimized new bioreactor production technology(experimental group Ⅱ)on the physicochemical indexes and microbial community of fermented grains were explored,and the correlation between the physicochemical indexes and microbial community structure of fermented grains in fermentation process was analyzed by re-dundancy analysis(RDA).The results showed that compared with the control group,the temperature of experimental group Ⅰ and Ⅱ rose faster,and the apex temperature was higher than that of the control group(30.0 ℃),which was 33.2 ℃ and 35.5 ℃,respectively.Experimental group Ⅱ had the optimal heating effect and kept the appropriate temperature for a longer time.The water content,starch and reducing sugar consumption of experimental group were higher,and the acidity of fermented grains in the lower layer of experimental group Ⅱ was the highest(4.24 mmol/10 g).Fermented grains in experimental group Ⅱ after fermentation,the relative abundance of dominant bacteria phylum Firmicutes and dominant fungi phylum Ascomycota was 92.44%and 97.59%,respectively.The relative abundance of dominant bacteria genus Lactobacillus was the highest(92.30%).The results of RDA showed that there was a significant correlation between the physicochemical index of fermented grains in experimental group Ⅱ and the changes of microbial community structure(P<0.05).

关键词

新型生物反应器/浓香型白酒/酒醅/微生物群落/理化指标/相关性

Key words

new bioreacter/strong-flavor Baijiu/fermented grains/microbial community/physicochemical index/correlation

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基金项目

酿酒生物技术及应用四川省重点实验室项目(NJ-2022-05)

中国轻工业酿酒生物技术及智能制造重点实验室项目(2023-04)

出版年

2024
中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
参考文献量37
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