首页|洞酿酱香型白酒第三轮次酒醅发酵过程中微生物与挥发性风味物质相关性分析

洞酿酱香型白酒第三轮次酒醅发酵过程中微生物与挥发性风味物质相关性分析

扫码查看
该研究应用Illumina MiSeq高通量测序技术和顶空固相微萃取-气相色谱质谱联用(HS-SPME-GC-MS)技术解析洞酿酱香型白酒第三轮次酒醅发酵过程中微生物多样性与挥发性风味物质变化。结合偏最小二乘法-判别分析(PLS-DA)筛出关键差异风味物质,并揭示优势菌属与挥发性风味物质的相关性。结果表明,所有酒醅样品共检测出4个优势菌门,16个优势菌属(包含8个细菌属和8个真菌属)。第三轮次酒醅发酵过程样品中共检出68种挥发性风味物质,其中醇类15种,酯类27种,醛类10种,酸类4种,酚类4种,其他类8种。PLS-DA模型共筛出关键差异风味物质16种。相关性结果表明,曲霉属(Aspergillus)与大多数酯类物质呈极显著正相关(P<0。01),Priceomyces和乳杆菌属(Lactobacillus)与十七烷酸乙酯呈极显著正相关(P<0。01)。
Correlation analysis of microorganism and volatile flavor substance of the third round fermented grains of Dongniang sauce-flavor Baijiuduring fermentation
In this study,the changes of microbial diversit y and volatile flavor substances during the third round of fermented grains of Dongniang sauce-flavor Baijiu were analyzed by Illumina MiSeq high-throughput sequencing technology and headspace solid phase microextraction gas chro-matography and mass spectrometry(HS-SPME-GC-MS).Key differential flavor substances were screened by partial least square-discriminant analysis(PLS-DA),and the correlation between dominant microorganism and volatile flavor substances was revealed.The results showed that 4 dominant phyla and 16 dominant genera(including 8 bacteria and 8 fungi)were detected in all the fermented grains samples.A total of 68 volatile flavor substances in the third round fermented grains of Dongniang sauce-flavor Baijiu were detected,including 15 alcohols,27 esters,10 aldehydes,4 acids,4 phenols and 8 others.16 key differential flavor substances were screened out by the PLS-DA model.The correlation results showed that Aspergillus showed highly significant positive correlation(P<0.01)with most esters,and Priceomyces and Lactobacillus showed highly significant positive correlation(P<0.01)with ethyl heptadecanoate.

Dongniangsauce-flavor Baijiufermented grainsmicroorganismvolatile flavor substancescorrelation

陈卓、苏伟、母应春、齐琦、邹恬杏、任婷婷、潘和勇、王家琴

展开 >

贵州大学 酿酒与食品工程学院,贵州 贵阳 550025

贵州大学 贵州省发酵工程与生物制药重点实验室,贵州 贵阳 550025

贵州洞酿洞藏酒业有限公司,贵州 遵义 564622

洞酿 酱香型白酒 酒醅 微生物 挥发性风味物质 相关性

贵州省科研单位服务企业项目

黔科合服企20204009006

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(5)
  • 36