Extraction,purification and structure identification of polysaccharides from Shanxi aged vinegar
Using Shanxi aged vinegar as raw material,the polysaccharide was extracted by ethanol precipitation method.The extraction conditions of polysaccharide in Shanxi aged vinegar(SAVP)were optimized by single factor and response surface tests,the refined polysaccharide and polysaccha-ride components were obtained by deproteinization,decolorization,dialysis,separation and purification with chromatographic column of crude polysaccharide.The structure of polysaccharide was identified by infrared spectroscopy,and the molecular weight,monosaccharide composition and thermogravimetric analysis were performed.The structure of the purified polysaccharide was preliminarily identified by infrared spectroscopy,and the molecular mass,monosaccharide composition and thermogravimetric analysis were performed.The results showed that the optimal conditions for alcohol precipitation were ethanol volume fraction 91%,alcohol precipitation time 16.3 h and alcohol multiple 4 times.Under the optimized condi-tions,the extraction amount of crude polysaccharide was 16.48 mg/g.The protein removal rate of crude polysaccharide by Sevag-trichloroacetic acid(TCA)method was 74.65%,and the polysaccharide retention rate was 77.87%.The decolorization rate of macroporous resin method was 64.02%and the retention rate of polysaccharide was 84.55%.The polysaccharide fraction SAVP-1 was obtained by purified with DEAE-52 cellulose and Sephadex G-100 column chromatography of refined polysaccharide.Structural analysis showed that SAVP-1 was α-type polysaccharide with a molecular mass of 6.87 kDa,which was consisted of 6 monosaccharides:rhamnose,arabinose,galactose,glucose,xylose,and mannose,and had bet-ter thermal stability.
Shanxi aged vinegarpolysaccharideextractionseparation and purificationstructural identification
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