Screening of fermentation strains of traditional Chinese medicine residue from Xiasangju and fermentation conditions optimization
Using the total alkaloid content as the evaluation index,the strains that increased the total alkaloid content in raw material of Xiasangju tra-ditional Chinese medicine residue were isolated and selected from the long-term stacking of Xiasangju traditional Chinese medicine residue,and identified by morphological characteristics,physiological and biochemical tests,and 16S rDNA sequence analysis,and the fermentation conditions of the strains were optimized through single-factor experiments and response surface methodology.The results showed that a total of 6 strains(L-1-L-6)that could increase the total alkaloid contents in Xiasangju traditional Chinese medicine residue were isolated and purified.Among them,the total al-kaloid content in the fermentation material by strain L-1 was the highest(24.89 mg/g)and the strain was identified as Bacillus subtilis.The optimal fermentation conditions were:material(Xiasangju traditional Chinese medicine residue 80 g+wheat bran 10 g+soybean cake powder 10 g)and water ratio 1∶1(g∶mL),loading volume 50 g/250 ml,inoculum 6.7%,fermentation time 6 d,and initial pH 6.5.Under these optimal conditions,the total alka-loid content reached 31.15 mg/g.
traditional Chinese medicine residueBacillus subtillisXiasangjutotal alkaloidsresponse surface optimization
万方数据
维普
