Using the total alkaloid content as the evaluation index,the strains that increased the total alkaloid content in raw material of Xiasangju tra-ditional Chinese medicine residue were isolated and selected from the long-term stacking of Xiasangju traditional Chinese medicine residue,and identified by morphological characteristics,physiological and biochemical tests,and 16S rDNA sequence analysis,and the fermentation conditions of the strains were optimized through single-factor experiments and response surface methodology.The results showed that a total of 6 strains(L-1-L-6)that could increase the total alkaloid contents in Xiasangju traditional Chinese medicine residue were isolated and purified.Among them,the total al-kaloid content in the fermentation material by strain L-1 was the highest(24.89 mg/g)and the strain was identified as Bacillus subtilis.The optimal fermentation conditions were:material(Xiasangju traditional Chinese medicine residue 80 g+wheat bran 10 g+soybean cake powder 10 g)and water ratio 1∶1(g∶mL),loading volume 50 g/250 ml,inoculum 6.7%,fermentation time 6 d,and initial pH 6.5.Under these optimal conditions,the total alka-loid content reached 31.15 mg/g.
traditional Chinese medicine residueBacillus subtillisXiasangjutotal alkaloidsresponse surface optimization
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