首页|青芒果-木瓜混合果皮酵素发酵工艺优化及其抗氧化活性

青芒果-木瓜混合果皮酵素发酵工艺优化及其抗氧化活性

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以植物乳杆菌(Lactobacillus plantarum)为发酵菌株,制备青芒果-木瓜混合果皮酵素。在单因素试验基础上,利用响应面法优化其发酵工艺条件。并通过考察1,1-二苯基-2-三硝基苯肼(DPPH)、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)、羟基自由基清除率和超氧化物歧化酶(SOD)活力评价其抗氧化性。结果表明,青芒果-木瓜混合果皮酵素最佳发酵工艺条件为发酵温度36℃、发酵时间60 h、菌种添加量5%、青芒果皮占比80%。在此优化条件下,酵素的ABTS、DPPH、羟基自由基清除率分别为92。42%、45。49%、69。85%,SOD活力为3 699。87 U/mL、总酚含量为4。09 mg/mL、总黄酮含量为5。10 mg/mL、维生素C含量为2。40 mg/mL。结果显示,青芒果-木瓜混合果皮酵素具有良好的抗氧化活性。
Optimization of fermentation process and antioxidant activity of Jiaosu with mixed peels of green mango and papaya
Jiaosu with mixed peels of green mango and papaya was prepared with Lactobacillus plantarum as fermentation strain.The fermentation pro-cess conditions were optimized by response surface method on the basis of single factor tests,and its antioxidant activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)(ABTS),hydroxyl radical scavenging rates and total superoxide dis-mutase(SOD)activity.The results showed that the optimum fermentation conditions of the Jiaosu were as follows:fermentation temperature 36℃,time 60 h,inoculum 5%and the ratio of green mango peel 80%.Under the optimized conditions,the ABTS,DPPH and hydroxyl radical scavenging rates of the Jiaosu were 92.42%,45.49%and 69.85%,the SOD activity of the Jiaosu was 3 699.87 U/ml,the contents of total phenols,total flavonoids and vitamin C of the Jiaosu were 4.09 mg/ml,5.10 mg/ml and 2.40 mg/ml,respectively.The results showed that Jiaosu with mixed peels of green mango and papaya had good antioxidant activity.

green mango peelpapaya peelJiaosu with mixed peelsfermentation processresponse surface optimization

商卓、刘杰文、刘红、高凤雅、杨定国、韩长日

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海南师范大学 化学与化工学院 海南 海口 571158

海口市热带特色药食同源植物研究与开发重点实验室,海南 海口 571158

海南科技职业大学 药食同源植物资源海南省重点实验室,海南 海口 571126

青芒果皮 木瓜皮 混合果皮酵素 发酵工艺 响应面优化

海口市重点科技计划大学生创新训练项目

2021034S202211658040

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(5)
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