中国酿造2024,Vol.43Issue(5) :144-149.DOI:10.11882/j.issn.0254-5071.2024.05.022

青芒果-木瓜混合果皮酵素发酵工艺优化及其抗氧化活性

Optimization of fermentation process and antioxidant activity of Jiaosu with mixed peels of green mango and papaya

商卓 刘杰文 刘红 高凤雅 杨定国 韩长日
中国酿造2024,Vol.43Issue(5) :144-149.DOI:10.11882/j.issn.0254-5071.2024.05.022

青芒果-木瓜混合果皮酵素发酵工艺优化及其抗氧化活性

Optimization of fermentation process and antioxidant activity of Jiaosu with mixed peels of green mango and papaya

商卓 1刘杰文 1刘红 2高凤雅 1杨定国 3韩长日3
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作者信息

  • 1. 海南师范大学 化学与化工学院 海南 海口 571158
  • 2. 海南师范大学 化学与化工学院 海南 海口 571158;海口市热带特色药食同源植物研究与开发重点实验室,海南 海口 571158
  • 3. 海南科技职业大学 药食同源植物资源海南省重点实验室,海南 海口 571126
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摘要

以植物乳杆菌(Lactobacillus plantarum)为发酵菌株,制备青芒果-木瓜混合果皮酵素.在单因素试验基础上,利用响应面法优化其发酵工艺条件.并通过考察1,1-二苯基-2-三硝基苯肼(DPPH)、2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS)、羟基自由基清除率和超氧化物歧化酶(SOD)活力评价其抗氧化性.结果表明,青芒果-木瓜混合果皮酵素最佳发酵工艺条件为发酵温度36℃、发酵时间60 h、菌种添加量5%、青芒果皮占比80%.在此优化条件下,酵素的ABTS、DPPH、羟基自由基清除率分别为92.42%、45.49%、69.85%,SOD活力为3 699.87 U/mL、总酚含量为4.09 mg/mL、总黄酮含量为5.10 mg/mL、维生素C含量为2.40 mg/mL.结果显示,青芒果-木瓜混合果皮酵素具有良好的抗氧化活性.

Abstract

Jiaosu with mixed peels of green mango and papaya was prepared with Lactobacillus plantarum as fermentation strain.The fermentation pro-cess conditions were optimized by response surface method on the basis of single factor tests,and its antioxidant activities were evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)(ABTS),hydroxyl radical scavenging rates and total superoxide dis-mutase(SOD)activity.The results showed that the optimum fermentation conditions of the Jiaosu were as follows:fermentation temperature 36℃,time 60 h,inoculum 5%and the ratio of green mango peel 80%.Under the optimized conditions,the ABTS,DPPH and hydroxyl radical scavenging rates of the Jiaosu were 92.42%,45.49%and 69.85%,the SOD activity of the Jiaosu was 3 699.87 U/ml,the contents of total phenols,total flavonoids and vitamin C of the Jiaosu were 4.09 mg/ml,5.10 mg/ml and 2.40 mg/ml,respectively.The results showed that Jiaosu with mixed peels of green mango and papaya had good antioxidant activity.

关键词

青芒果皮/木瓜皮/混合果皮酵素/发酵工艺/响应面优化

Key words

green mango peel/papaya peel/Jiaosu with mixed peels/fermentation process/response surface optimization

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基金项目

海口市重点科技计划(2021034)

大学生创新训练项目(S202211658040)

出版年

2024
中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
参考文献量27
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