In order to further clarify the labeling of animal and plant-derived allergens in commercially available food,a multiplex ligation-dependent probe amplification(MLPA)technique was developed,which could simultaneously detect 21 animal-and plant-derived allergens in one reaction.The specificity and sensitivity of the newly established MLPA technique was analyzed.The animal-and plant-derived allergens in 38 commercially available pre-packaged foods were compared and analyzed using MLPA technique and real-time fluorescent quantitative polymerase chain reaction(RT-fqPCR)detection technology.The results showed that 21 species of animal and plant allergens could be detected by MLPA at the same time,there was no cross interference between amplification peaks,the difference between the actual and theoretical amplification peaks was less than 3 bp.The minimum detectable deoxyribonucleic acid(DNA)concentration was 1 ng/μl.The detection rates of RT-fqPCR and MLPA were 51.5%and 44.1%,respectively.In addition,the allergen components in 6 samples labeled with possible allergens and 10 samples with unlabeled allergens were detected by MLPA.Therefore,the sensitivity of MLPA technique to detect 21 animal and plant allergens was higher.
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