Sleep aid function and safety of complex healthy liquor of spina date seed based on zebrafish model
In order to study the sleep aid effect and safety of complex healthy liquor of spina date seed,using the zebrafish 5 d after fertilization as the experimental subjects,a zebrafish insomnia model was established by administering p-pentatetrazole(PTZ)by water solution.The waking activity and total waking time of model zebrafish and normal zebrafish were measured by behavior analyzer,and the sleep improving effect of the liquor was eval-uated.Taking zebrafish 2 d after fertilization as the experiment object,the safety of the liquor was evaluated by the incidence of tissue and organ toxi-city of zebrafish.The results showed that the liquor had the effect of improving the sleep of model zebrafish and normal zebrafish,which showed as follows:significantly decreased the waking activity and the total waking time of model zebrafish(P＜0.05),and decreased the waking activity and the total waking time of normal zebrafish.The results of acute toxicity test of zebrafish showed that the liquor was safe to zebrafish at administration of 2.83 μl/ml,and toxic to zebrafish when the administration exceeded 8.48 μl/ml.To sum up,the complex healthy liquor of spina date seed had the ef-fect of sleep aid function on zebrafish.
spina date seedcomplex healthy liquorsleep aidsafetyzebrafish
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