Optimization of fermentation process of exopolysaccharide production by Levilactobacillus brevis M-10
Using exopolysaccharide(EPS)producing Levilactobacillus brevis M-10 isolated from Northwestern Shanxi sour porridge as research object,the fermentation process of L.brevis M-10 for EPS production was optimized by single factor and response surface experiments based on the opti-mized carbon source and nitrogen source using MRS medium.The results showed that the fermentation temperature had an extremely significant ef-fect on the EPS production of L.brevis M-10(P＜0.01),and the inoculum had a significant effect(P＜0.05).The optimal fermentation process was obtained as follows:fermentation temperature 36℃,time 45 h,initial pH 6.2 and inoculum 1.0％(V/V).Under these optimal conditions,the EPS yield was(2.54±0.13)g/L,which was 2.42 times higher than that before optimization.
NETL
NSTL
万方数据
