Optimization of conditions for high-yield cellulase by 2 fungal strains mixed fermentation
The existing agricultural waste treatment methods can not effectively degrade cellulose in straw,resulting in a large amount of straw waste.In order to better apply microbial fermentation in industrial production,improve the cellulase yield and utilization rate of agricultural straw waste,liquid fermentation was carried out with 2 strains of fungi Irpex lacteus J2 and Trametes junipericola J6 as mixed strains.Using cellulase activity as the response value,the composition of cellulose-producing medium was optimized by Plackett-Burman tests,the steepest climb tests and response surface tests,and the fermentation conditions were optimized by single factor tests.The results showed that the optimal cellulase-producing medium composition was lactose 10.1 g/L,beef paste 3 g/L,KH2PO4 4.4 g/L,MgSO4 0.3 g/L,MnSO4 0.3 g/L and Tween-80 1.5 ml/L.The optimal culture conditions were as follows:initial pH 6.0,inoculum ratio of strain J2 and J6 1∶1,fermentation temperature 28 ℃,fermentation time 4 d,rotation speed 170 r/min.Under these optimal conditions,the highest cellulase activity was 112.1 U/ml,which was 275.8％higher than that before optimiza-tion(29.83 U/ml).The experimental results could provide some support for the further application of fungal mixed fermentation in cellulase production,provide basic data for industrial production,and lay a foundation for green,safe and efficient treatment of agricultural waste.
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