为了解明胶加工废水处理过程中的细菌菌群结构差异性,采用Illumina Hiseq 2500平台对初沉水、好氧水、二沉泥和排放水4个取样点废水的细菌16S rRNA V3～V4区进行高通量测序.结果表明,在门水平,初沉水的优势细菌为变形菌门(Proteobacteria)(69.62％)和厚壁菌门(Firmicutes)(29.77％),好氧水和二沉泥的优势细菌为uncultured bacterium WS6(34.48％、22.75％)和变形菌门(24.85％、22.88％),排放水的优势细菌为螺旋体菌门(Saccharibacteria)(8.89％)和变形菌门(16.49％).在属水平,初沉水的优势细菌为噬氢菌属(Hydrogenophaga)(37.57％),好氧水和二沉泥的优势细菌为uncultured bacterium p WS6(34.48％、22.76％),排放水的优势细菌为uncultured bacterium p Saccharibacteria(28.89％).不同废水处理单元的微生物菌群结构差异明显.废水中的细菌群落与水质环境因子之间存在显著相关性(P＜0.05).海默氏菌属(Rheinheimera)与pH、Ca2+和氨氮浓度呈显著正相关(P＜0.05),嗜碱菌属(Alka-libacterium)与总氮和化学需氧量呈显著正相关(P＜0.05).总之,废水水体环境因子与微生物菌群结构密切相关,通过构建二者之间关联性可了解废水中关键微生物菌群的功能作用.
Analysis of microbial community differences during wastewater treatment of gelatin production plants based on high-throughput sequencing
In order to understand the structural differences of bacterial flora in the treatment process of gelatin processing wastewater,the 16S rRNA V3-V4 region of bacterial community in four sampling points of wastewater(initial settling water,aerobic water,secondary sedimentary sludge and discharge water)were investigated by high throughput sequencing using Illumina Hiseq sequencing platform.The results demonstrated that the domi-nant bacteria of initial settling water were Proteobacteria(69.62％)and Firmicutes(29.77％)at phylum level,Uncultured bacterium WS6(34.48％,22.75％)and Proteobacteria(24.85％,22.88％)were dominated in aerobic water and secondary sedimentary sludge,Saccharibacteria(8.89％)and Proteobacteria(16.49％)were dominated in discharge water.At the genus level,the dominant bacteria of initial settling water were Hydrogenophaga(37.57％),while the dominant bacteria in aerobic wastewater and secondary sedimentary sludge were uncultured bacterium p WS6(34.48％,22.76％),and the dominant bacteria of discharge water was uncultured bacterium p Saccharibacteria(28.89％).There were significant differences of bacterial community diversity of four wastewater samples.The bacterial communities structure in wastewater treatment system was significantly correlated with water quality environmental factors(P＜0.05).Rheinheimera was positively correlated with pH and the content of Ca2+,ammonia nitrogen(P＜0.05).Alkalibacterium was positively correlated with the content of total nitrogen and chemical oxygen demand(P＜0.05).In summary,the environ-mental factors of wastewater were closely related to the structure of microbial communities,and the functions of key bacterial community in wastew-ater treatment was ascertained by the established relationship between environmental factors and microbial communities.
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