Optimization of fermentation conditions of lipopeptide-producing Bacillus amyloliquefaciens HY2-1
Bacillus amyloliquefaciens HY2-1 is the previously isolated marine microorganism that can produce lipopeptides such as fengycins and sur-factants.In order to improve the antimicrobial activity of lipopeptides by strain HY2-1,using the bacterial inhibition zone diameter as response value,the carbon and nitrogen sources of the fermentation medium and fermentation conditions of strain HY2-1 were optimized by single-factor and response surface test design.The results showed that the optimum carbon and nitrogen sources for the fermentation medium of strain HY2-1 were soluble starch 80 g/L,yeast extract powder and peptone(1∶1)10 g/L,and the optimal fermentation conditions were loading volume 50 ml/250 ml,fermentation time 48 h,inoculum 1％and rotation speed 200 r/min.Under these conditions,the inhibitory circle diameter of strain HY2-1 fermentation broth on Penicillium digitatum was(18.6±0.1)mm,which was 26.5％higher than that ofbefore optimization(14.7±0.5)mm.Through the optimization of carbon and nitrogen sources of fermentation medium and fermentation conditions,the antibacterial inhibitory activity of HY2-1 lipopeptides was significantly improved.
Bacillus amyloliquefacienslipopeptidesfermentation mediumfermentation conditionresponse surface test
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