脂肽产生菌解淀粉芽孢杆菌HY2-1的发酵条件优化
Optimization of fermentation conditions of lipopeptide-producing Bacillus amyloliquefaciens HY2-1
廖凤平 1李昆太 1谭明辉 1黎循航2
作者信息
- 1. 广东海洋大学食品科技学院,广东湛江 524088;广东省水产品加工与安全重点实验室,广东湛江 524088;广东省海洋食品工程技术研究中心,广东湛江 524088
- 2. 豫章师范学院小学教育学院,江西南昌 330103
- 折叠
摘要
解淀粉芽孢杆菌(Bacillus amyloliquefaciens)HY2-1系前期筛选到的产芬原素和表面活性素等脂肽类物质的海洋微生物.为提高菌株HY2-1脂肽类抑菌活性,以菌株抑菌圈直径为响应值,通过单因素和响应面试验设计对菌株HY2-1的发酵培养基碳氮源及发酵条件进行优化.结果表明,菌株HY2-1发酵培养基最佳碳氮源为可溶性淀粉80g/L,酵母浸粉和蛋白胨(1∶1)10g/L,最佳发酵条件为:装液量50 mL/250 mL,发酵时间48 h,接种量1％,转速200 r/min,在此条件下其发酵液对指状青霉的抑菌圈直径为(18.6±0.1)mm,较优化前(14.7±0.5)mm提高26.5％;该研究通过发酵培养基碳氮源及发酵条件优化,对提高菌株HY2-1脂肽抑菌活性效果显著.
Abstract
Bacillus amyloliquefaciens HY2-1 is the previously isolated marine microorganism that can produce lipopeptides such as fengycins and sur-factants.In order to improve the antimicrobial activity of lipopeptides by strain HY2-1,using the bacterial inhibition zone diameter as response value,the carbon and nitrogen sources of the fermentation medium and fermentation conditions of strain HY2-1 were optimized by single-factor and response surface test design.The results showed that the optimum carbon and nitrogen sources for the fermentation medium of strain HY2-1 were soluble starch 80 g/L,yeast extract powder and peptone(1∶1)10 g/L,and the optimal fermentation conditions were loading volume 50 ml/250 ml,fermentation time 48 h,inoculum 1％and rotation speed 200 r/min.Under these conditions,the inhibitory circle diameter of strain HY2-1 fermentation broth on Penicillium digitatum was(18.6±0.1)mm,which was 26.5％higher than that ofbefore optimization(14.7±0.5)mm.Through the optimization of carbon and nitrogen sources of fermentation medium and fermentation conditions,the antibacterial inhibitory activity of HY2-1 lipopeptides was significantly improved.
关键词
解淀粉芽孢杆菌/脂肽类物质/发酵培养基/发酵条件/响应面试验Key words
Bacillus amyloliquefaciens/lipopeptides/fermentation medium/fermentation condition/response surface test引用本文复制引用
基金项目
广东海洋大学科研启动项目(060302042006)
广东省普通高校重点领域专项(2021ZDZX4010)
出版年
2024
NETL
NSTL
万方数据