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猕猴桃酵素发酵工艺优化及体外抗氧化活性测定

Optimization of fermentation process and determination of antioxidant activity in vivo of kiwifruit Jiaosu

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为进一步延伸猕猴桃加工链,该研究以猕猴桃为原料,通过单因素试验、Plackett-Burman试验和响应面试验优化猕猴桃酵素发酵工艺,并分析猕猴桃酵素对2,2'-联氮-双-3-乙基苯并噻唑啉-6-磺酸(ABTS+)、1,1-二苯基-2-三硝基苯肼(DPPH)、羟基自由基清除效果及Fe3+的相对还原能力.结果表明,猕猴桃酵素最佳发酵工艺为料液比38∶100(g∶mL)、菌种(酿酒酵母∶植物乳杆菌∶保加利亚乳杆菌=3∶1∶1)接种量0.24%、白砂糖添加量12%,发酵时间144h,发酵温度26.4℃.在最佳工艺条件下制备的猕猴桃酵素超氧化物歧化酶(SOD)酶活力可达1 893.30 U/mL.抗氧化试验结果表明,猕猴桃酵素对DPPH·、ABTS+·、·OH清除率及Fe3+相对还原能力分别为37.2%、80.13%、79.01%及24.65%;分别比优化前提高了7.98%、2.50%、2.41%及3.58%.综上,经过发酵工艺优化后猕猴桃酵素SOD酶活增加,抗氧化活性有所提升.
In order to further prolong the processing industry of kiwifruit,taking kiwifruit as raw material,the fermentation conditions of kiwifruit Jiaosu were optimized by single factor tests,Plackett-Burman tests and response surface tests,and the removal effect of kiwifruit Jiaosu on 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS+),1,1-diphenyl-2-picrylhydrazyl(DPPH),hydroxyl free radical and Fe3+relative reducing capacity were analyzed.The results showed that the optimal fermentation process of kiwifruit Jiaosu was as follows:solid to liquid ratio 38∶100(g∶ml),bacteria(Saccharomyces cerevicae∶Lactobacillus plantarum∶Lactobacillus bulgaricus=3∶1∶1)inoculum 0.24%,sugar addition 12%,fermentation time 144 h,temperature 26.4 ℃.The superoxide dismutase(SOD)activity of the kiwifruit Jiaosu under the optimal conditions could reach 1 893.30 U/ml.The re-sults of antioxidant tests showed that the clearance rates of DPPH·,ABTS+·,·OH and Fe3+relative reducing capacity were 37.2%,80.13%,79.01%and 24.65%,respectively,which improved 7.98%,2.50%,2.41%and 3.58%respectively compared with that of before optimization.In conclusion,the SOD activity of the kiwifruit Jiaosu and the antioxidant activity were improved after fermentation process optimization.

kiwifruitJiaosuantioxidant activityprocess optimization

王荣荣、刘晓媛、涂剑秋

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信阳农林学院食品科学与工程学院,河南信阳 464000

猕猴桃 酵素 抗氧化活性 工艺优化

河南省高等学校青年骨干教师培养计划信阳农林学院青年基金项目河南省高等学校重点科研项目

2020GGJS262QN202303124B550016

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(7)
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