Simultaneous determination of 11 synthetic colorants in wine by HPLC
Aiming to quickly identify whether synthetic colorants are added to wine and accurately quantify,a HPLC method was established to simul-taneously detect 11 kinds of synthetic colorants in wine.The experimental samples were diluted with ammonium acetate solution,filtered directly after centrifugation at 8 000 r/min,separated with Zorbax SB-C18 column by gradient elution,and detected by multi-wavelength(288 nm,425 nm,515 nm,620 nm).The results shown that the method was characterized by simple pre-treatment,no interference from impurities under the maximum absorp-tion wavelength of each colorants,high sensitivity,low detection limit(0.05 mg/L),low limit of quantitation(0.15 mg/L),and good linear relationship(R2≥ 0.999 4)in the range of 0.20-50 mg/L.The relative standard deviation(RSD)of precision tests results was 1.86％-2.61％,and the recovery rates were 93.0％-103.0％.The method was suitable for rapid and accurate detection of synthetic colorants in wine.
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