高效液相色谱法同时检测葡萄酒中11种人工合成着色剂
Simultaneous determination of 11 synthetic colorants in wine by HPLC
李广 1李琪 1顾敏 1张锋1
作者信息
- 1. 甘肃省分析测试中心,甘肃兰州 730000
- 折叠
摘要
为快速识别葡萄酒中是否添加人工合成着色剂并准确定量,该实验样品采用乙酸铵溶液稀释、8000r/min离心后直接过滤法进行前处理,梯度洗脱、Zorbax SB-C18柱分离、多波长检测(288 nm、425 nm、515 nm、620 nm),建立了高效液相色谱法(HPLC)同时检测葡萄酒中11种人工合成着色剂的方法.结果表明,该方法前处理简单,各着色剂最大吸收波长检测无杂质干扰、灵敏度高、检出限(0.05 mg/L)、定量限(0.15 mg/L)低,在质量浓度0.20～50 mg/L范围内线性关系良好(R2≥0.999 4),方法精密度试验结果的相对标准偏差(RSD)为1.86％～2.61％,加标回收率为93.0％～103.0％.适用于葡萄酒中人工合成着色剂的快速、准确检测.
Abstract
Aiming to quickly identify whether synthetic colorants are added to wine and accurately quantify,a HPLC method was established to simul-taneously detect 11 kinds of synthetic colorants in wine.The experimental samples were diluted with ammonium acetate solution,filtered directly after centrifugation at 8 000 r/min,separated with Zorbax SB-C18 column by gradient elution,and detected by multi-wavelength(288 nm,425 nm,515 nm,620 nm).The results shown that the method was characterized by simple pre-treatment,no interference from impurities under the maximum absorp-tion wavelength of each colorants,high sensitivity,low detection limit(0.05 mg/L),low limit of quantitation(0.15 mg/L),and good linear relationship(R2≥ 0.999 4)in the range of 0.20-50 mg/L.The relative standard deviation(RSD)of precision tests results was 1.86％-2.61％,and the recovery rates were 93.0％-103.0％.The method was suitable for rapid and accurate detection of synthetic colorants in wine.
关键词
人工合成着色剂/葡萄酒/直接过滤/检测/高效液相色谱Key words
synthetic colorants/wine/direct filtration/determination/HPLC引用本文复制引用
基金项目
甘肃省属科研院所创新能力建设专项(21JR7RA765)
出版年
2024
NETL
NSTL
万方数据