首页|混菌发酵条件下酵母对葡萄酒中赭曲霉毒素A的去除研究

混菌发酵条件下酵母对葡萄酒中赭曲霉毒素A的去除研究

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为探究本土酿酒酵母(Saccharomyces cerevisiae)(C)及混菌发酵对葡萄酒中赭曲霉毒素A(OTA)的去除及机理,将其与3株非酿酒酵母(N、I、K)组合进行混菌发酵,比较不同组合对葡萄酒中OTA的去除情况;通过酵母胞内外产物及酵母细胞特性探究酵母对OTA的去除机理。结果表明,4株酵母的OTA耐受能力较好,混合发酵C-K组的OTA去除率(24。09%)显著高于其他组(P<0。05);4组红葡萄酒(C、C-N、C-I、C-K)酿造完成后OTA含量均处于安全范围,其中主发酵阶段OTA含量变化最大(47。41%~63。02%),其次为皮渣分离后和陈酿后;由于酿酒酵母C的细胞壁较厚(192。76 nm),对OTA吸附能力较强,因此其单独发酵时OTA的去除率高达63。02%。该研究探究了本土酿酒酵母及混菌发酵对葡萄酒中OTA的去除情况,为葡萄酒中OTA含量控制提供了参考。
Removal of ochratoxin A from wine by yeast under mixed-strain fermentation conditions
In order to explore the ochratoxin A(OTA)reduction and mechanism from wine by native Saccharomyces cerevisiae(C)and mixed-strain fermentation,three non-S.cerevisiae strains(N,I,K)were combined for mixed fermentation,and the OTA removal efficiency by different yeast com-binations in wine was compared.The mechanism of OTA removal by yeast was investigated through the characteristics of yeast cell and intracellular products.The results showed that the 4 strains of yeast had better OTA tolerance,and the OTA removal rate in mixed fermentation C-K group(24.09%)was significantly higher than that in other groups(P<0.05).The OTA content of the four groups of red wines(C,C-N,C-I,C-K)was in the safe range after brewing,and the OTA content of the main fermentation stage changed the most(47.41%-63.02%),followed by the separation of skin residue and aging.Due to the cell wall of S.cerevisiae C was thicker(192.76 nm)and the adsorption capacity of OTA was strong,the OTA removal rate of S.cerevisiae C was 63.02%during single fermentation.This study investigated the removal of OTA from wine by domestic yeast and mixed-strains fermentation,which provided a reference for controlling OTA content in wine.

native Saccharomyces cerevisiaefermentationwineochratoxin A removal

刘圆圆、刘一辉、李强、李华、刘满顺、王华

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西北农林科技大学 葡萄酒学院,陕西杨凌 712100

宁夏回族自治区食品检测研究院,宁夏银川 750001

本土酿酒酵母 发酵 葡萄酒 赭曲霉毒素A去除

西北农林科技大学博士科研启动经费资助国家市场监管重点实验室开放课题资助

Z10901220282023KF0001

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(8)