首页|内蒙古地区自然发酵酸菜汤中亚硝酸盐、有机酸含量和细菌群落特征分析

内蒙古地区自然发酵酸菜汤中亚硝酸盐、有机酸含量和细菌群落特征分析

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利用高效液相色谱技术和高通量测序技术对内蒙古10个地区(编号分别为HS、WLCB、CF、BT、BM、ALS、HM、ERDS、XAM和TL)自然发酵酸菜汤样品中亚硝酸盐、有机酸含量和细菌群落特征进行分析,并对结果进行相关性分析。结果表明,自然发酵酸菜汤样品中亚硝酸盐含量在0。04~0。28 mg/kg之间。乳酸是含量最高的有机酸(1。97~46。45 mg/mL),草酸、甲酸、苹果酸、乙酸、柠檬酸和富马酸含量在各样品间差异显著(P<0。05)。所采集样品中厚壁菌门(Firmictes)和变形菌门(Proteobacteria)是优势细菌门,乳杆菌属(Lactobacillus)和片球菌属(Pediococcus)为优势细菌属。基于群落结构差异,可将10份样品分为三个类型。相关性分析结果显示,乳球菌属(Lactococcus)和明串珠菌属(Leuconostoc)与多数细菌属呈显著正相关(P<0。05),Lactococcus、Leuconostoc和魏斯氏菌属(Weissella)等属与除乳酸和苹果酸以外的7种有机酸分别呈显著正相关(P<0。05),苹果酸与亚硝酸盐呈显著正相关(P<0。05)。
Analysis of nitrite,organic acid content and bacterial community characteristics in naturally fermented sauerkraut soup of Inner Mongolia
The characteristics of nitrites,organic acids,and bacterial communities were analyzed in naturally fermented sauerkraut soup from 10 regions(numbered as HS,WLCB,CF,BT,BM,ALS,HM,ERDS,XAM and TL)in Inner Mongolia by high performance liquid chromatography(HPLC)and high-throughput sequencing technology,and the correlation analysis of the results was conducted.The results showed that the nitrite content in naturally fermented sauerkraut soup ranged from 0.04 to 0.28 mg/kg.Lactic acid was the organic acid with the highest content(1.97-46.45 mg/ml),while oxalic acid,formic acid,malic acid,acetic acid,citric acid,and fumaric acid showed significant differences among the samples(P<0.05).Moreover,Firmicutes and Proteobacteria were dominant bacterial phyla,while Lactobacillus and Pediococcus were dominant bacterial genera in the collected samples.Based on differences in community structure,10 samples could be divided into three types.Correlation analysis showed that Lactococcus and Leuconostoc were significantly positively correlated with most bacterial genera(P<0.05),while Lactococcus,Leuconostoc and Weissella were significantly positively correlated with seven organic acids except for lactic acid and malic acid(P<0.05),and malic acid was significantly positively correlated with nitrite(P<0.05).

sauerkrautbacterial diversityorganic acidnitriteInner Mongolia region

许建洋、陈忠军、张作仪、赵锦、孙子羽、满都拉

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内蒙古农业大学 食品科学与工程学院,内蒙古呼和浩特 010018

酸菜 细菌多样性 有机酸 亚硝酸盐 内蒙古地区

国家自然科学基金地区科学基金项目内蒙古农业大学食品科学与工程学院科技项目

32060533SPKJ201912

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(8)