复合露酒的非挥发性成分鉴定及抗疲劳效应
Identification of non-volatile components and anti-fatigue effect of a compound Lujiu
陈颖 1朱禹哲 1余美丽 2刘方美 1李存玉 3郑云枫 3彭国平3
作者信息
- 1. 南京中医药大学 药学院,江苏 南京 210023
- 2. 江苏洋河酒厂股份有限公司,江苏 宿迁 223800
- 3. 南京中医药大学 药学院,江苏 南京 210023;中药制药过程控制与智能制造技术全国重点实验室,江苏连云港 222047
- 折叠
摘要
该研究采用高效液相色谱-四级杆飞行时间串联质谱联用(HPLC-Q-TOF-MS/MS)法对复合露酒(38％vol)中的非挥发性化合物进行检测,并将复合露酒灌胃至小鼠22 d,考察其对小鼠负重游泳的影响,评价其抗疲劳的保健作用.结果表明,从复合露酒(0.225 7g生药/mL)中共鉴定出52种非挥发性物质,包括皂苷类24种、黄酮类6种、核苷类4种、氨基酸类4种、生物碱类3种、其他11种.与模型组相比,低、中、高剂量组小鼠体质量减少量均极显著减少(P＜0.01);中、高剂量组小鼠负重游泳时间均极显著延长(P＜0.01);低、中剂量组小鼠血尿素氮(BUN)含量显著增加(P＜0.01,P＜0.05);低、中、高剂量组小鼠乳酸脱氢酶(LDH)活力、肝糖原(LG)含量显著增加(P＜0.01,P＜0.05),说明该款复合露酒非挥发性成分丰富,可增强身体对负荷的适应性,具有明显的抗疲劳效果,对功能性食品开发提供一定的基础.
Abstract
The non-volatile compounds in a compound Lujiu(38％vol)was detected by high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry(HPLC-Q-TOF-MS/MS).The compound Lujiu was intragastric administered to mice for 22 d to investigate its effect on weight-bearing swimming and evaluate its anti-fatigue health benefits.The results showed that a total of 52 non-volatile substances were identified from the compound Lujiu(0.225 7 g crude drug/ml),including 24 saponins,6 flavonoids,4 nucleosides,4 amino acids,3 alkaloids,and 11 others.Compared with the model group,the body weight reduction of mice in the low,medium and high dose groups was significantly reduced(P＜0.01),the weight-bearing swimming time of mice in the medium and high dose groups was significantly prolonged(P＜0.01),the content of blood urea nitrogen(BUN)of mice in low and medium dose groups was significantly increased(P＜0.01,P＜0.05),the activity of lactate dehydrogenase(LDH)and the content of liver glycogen(LG)of mice in the low,medium and high dose groups were significantly increased(P＜0.01,P＜0.05),indicating that the compound Lujiu was rich in non-volatile substances,which could enhance the body's adaptability to load and had a significant anti-fatigue effect,providing a certain foundation for the development of functional foods.
关键词
复合露酒/高效液相色谱-四级杆飞行时间串联质谱/非挥发性成分/抗疲劳Key words
compound Lujiu/high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry/non-volatile composition/anti-fatigue引用本文复制引用
基金项目
国家自然科学基金资助项目(82074006)
出版年
2024
国家科技期刊平台
NSTL
万方数据