Identification of non-volatile components and anti-fatigue effect of a compound Lujiu
The non-volatile compounds in a compound Lujiu(38％vol)was detected by high-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometry(HPLC-Q-TOF-MS/MS).The compound Lujiu was intragastric administered to mice for 22 d to investigate its effect on weight-bearing swimming and evaluate its anti-fatigue health benefits.The results showed that a total of 52 non-volatile substances were identified from the compound Lujiu(0.225 7 g crude drug/ml),including 24 saponins,6 flavonoids,4 nucleosides,4 amino acids,3 alkaloids,and 11 others.Compared with the model group,the body weight reduction of mice in the low,medium and high dose groups was significantly reduced(P＜0.01),the weight-bearing swimming time of mice in the medium and high dose groups was significantly prolonged(P＜0.01),the content of blood urea nitrogen(BUN)of mice in low and medium dose groups was significantly increased(P＜0.01,P＜0.05),the activity of lactate dehydrogenase(LDH)and the content of liver glycogen(LG)of mice in the low,medium and high dose groups were significantly increased(P＜0.01,P＜0.05),indicating that the compound Lujiu was rich in non-volatile substances,which could enhance the body's adaptability to load and had a significant anti-fatigue effect,providing a certain foundation for the development of functional foods.
compound Lujiuhigh-performance liquid chromatography quadrupole time-of-flight tandem mass spectrometrynon-volatile compositionanti-fatigue
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