Screening and identification of superior mold in Yilite strong-flavor Daqu and research on Fuqu-making technology
The molds with high saccharifying enzyme and liquefying enzyme activity were isolated and screened by traditional cultivation and separation methods from Yilite strong-flavor(Nongxiangxing)Daqu,and identified through morphological observation and molecular biology techniques.Fuqu was made using screened strains,and the Fuqu-making conditions were optimized by single factor experiments and response surface experiments us-ing saccharifying enzyme and liquefying enzyme activity as evaluation indicators.The results showed that a strain of mold with superior performance in producing saccharifying enzyme and liquefying enzymes was screened,numbered JW-3,and the strain JW-3 was identified as Lichtheimia corymb-ifera.The optimal Fuqu-making conditions for the production of pure strain Fuqu with L.corymbifera JW-3 were obtained as follows:inoculum 10％,culture temperature 30 ℃,time 91 h,and water content 55％.Under these optimal conditions,the saccharifying enzyme activity of pure mold Fuqu was 409.67 U/g,and the liquefying enzyme activity was 1 597.00 U/g,which had certain application potential in Baijiu brewing.
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