高产甾醇热带假丝酵母发酵工艺优化及富含甾醇调制乳的制备
Optimization of fermentation process of high-yield sterol Candida tropicalis and preparation of sterol-rich modified milk
杨婷婷 1曾秋月 1余春雨 1许鑫业 1田洋 2黄艾祥1
作者信息
- 1. 云南农业大学 食品科学技术学院,云南 昆明 650201
- 2. 云南农业大学 食品科学技术学院,云南 昆明 650201;食药同源资源开发与利用教育部工程研究中心,云南 昆明 650201;云南省药食同源功能食品工程研究中心,云南 昆明 650201;云南省精准营养与个性化食品制造重点实验室中心,云南 昆明 650201
- 折叠
摘要
该研究以脱脂奶粉及核桃油为主要原料,以高产甾醇热带假丝酵母(Candida tropicalis)1253、保加利亚乳杆菌(Lactobacillus bulgaricus)及嗜热链球菌(Streptococcus thermophilus)为发酵菌种制备甾醇冻干粉,并在此基础上研制富含甾醇调制乳.以甾醇产量为评价指标,通过单因素试验和响应面试验优化菌株1253发酵工艺,对富含甾醇调制乳品质和营养进行综合性评价,并分析其关键差异代谢物及代谢通路.结果表明,最佳发酵工艺为培养时间97 h,培养温度30 ℃,菌种接种量9.5％,核桃油添加量4％.在此优化条件下,甾醇产量为0.90 mg/g.富含甾醇调制乳感官评分为(86.44±2.06)分,甾醇含量为(22.7±0.6)mg/g.吡哆醛、胆甾醇、γ-氨基丁酸(GABA)、麦角甾苷和柠檬酸等关键差异代谢物通过富集维生素B6代谢、磷酸戊糖途径、乙氧基化物和二甲酸酯代谢、果糖和甘露糖代谢和柠檬酸循环等途径形成特征风味.
Abstract
In this study,using defatted milk powder and walnut oil as main raw materials,the freeze-dried sterol powder was prepared with high-yield sterol Candida tropicalis 1253,Lactobacillus bulgaricus and Streptococcus thermophilus as fermentation strains.On this basis,the sterol-rich modified milk was prepared.Using sterol yield as evaluation index,the fermentation process of strain 1253 was optimized by single factor tests and response surface tests.The quality and nutrition of sterol-rich modified milk was evaluated comprehensively,and the key differential metabolites and metabolic pathways were analyzed.The results showed that the optimum fermentation technology was culture time 97 h,temperature 30 ℃,inoculum 9.5％,walnut oil addition 4％.Under the optimized conditions,the sterol yield was 0.90 mg/g.The sensory score of sterol-enriched modified milk was(86.44±2.06)and the sterol content was(22.7±0.6)mg/g.Key differential metabolites such as pyridoxal,cholesterol,gamma-aminobutyric acid(GABA),acteoside and citric acid formed the characteristic flavors through enrichment of vitamin B6 metabolism,pentose phosphate pathway,ethoxylate and dicarboxy-late metabolism,fructose and mannose metabolism,and citric acid cycle.
关键词
高产甾醇热带假丝酵母/发酵工艺/富含甾醇调制乳/品质分析/差异代谢物Key words
high-yield sterol Candida tropicalis/fermentation process/sterol-rich modified milk/quality analysis/differential metabolites引用本文复制引用
基金项目
云南省重大科技项目(202102AE090027-1)
云南省省市一体化专项(A3032023057)
云岭产业技术领军人才项目(云发改人事[2014]1782号)
出版年
2024
国家科技期刊平台
NSTL
万方数据