In this study,yeasts were isolated from Macadamia ternifolia honey by traditional culture methods,identified by morphological observation and molecular biological technology,and its growth characteristics were studied.The results showed that 6 strains with ester flavor were isolated from 3 M.ternifolia honey samples,numbered as JG01-8,JG01-15,JG01-H4,JG02-9,JG02-11 and JG03-1,respectively.After identification,the strain JG01-8 was identified as Hannaella luteola,the strain JG01-H4 was identified as Sporobolomyces carnicolor,and the strains JG01-15、JG02-9、JG02-11 and JG03-1 were identified as Zygosaccharomyces rouxii,which were all non-Saccharomyces yeasts.The tolerance of strain JG01-H4 was better than others,which could tolerate ethanol volume fraction 9％,pH 2.0,NaCl content 25％and glucose content 60％,and it was potential strain for fermentation in high permeability environment.
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