Optimization of extraction and purification process of lycopene from engineered Saccharomyces cerevisiae and structure identification
In order to explore the extraction and purification method and structure of lycopene produced by engineered Saccharomyces cerevisiae,the extraction and purification technology of lycopene was optimized by single factor and orthogonal tests,and its structure was identified by nuclear magnetic resonance(NMR).The results showed that the optimum extraction conditions of lycopene were acid heat breaking wall:hydrochloric acid concentration 3.0 mol/L,hydrochloric acid dosage 3.0 ml/0.1 g yeast,acidification time 60 min,and temperature 45 ℃.NaOH degreasing:NaOH concentration 0.10 mol/L,NaOH dosage 2.0 ml/0.1 g yeast,saponification time 30 min,water bath temperature 40 ℃ after saponification.Acetone extraction:material-liquid ratio 1∶20(g∶ml),extraction temperature 40 ℃,and time 50 min.The optimal purification process was n-hexane crystal-lization:crude extract was dissolved and filtered with n-hexane at 40 ℃ and crystallized at-20 ℃ for 12 h.Under the optimum extraction and purifi-cation conditions,the extraction rate of lycopene was 76％and the purity of lycopene was 96.7％.NMR results showed that the structure of lycopene was all-trans lycopene,which was consistent with plant-derived lycopene.
engineered Saccharomyces cerevisiaelycopeneextraction and purificationprocess optimizationstructure identification
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