Optimization of high-density culture conditions for Weizmannia coagulans
In order to meet the increasing market demand for Weizmannia coagulans and obtain a method for high-density culture of W.coagulans,using W.coagulans D1 as the experimental object,based on MRS medium,the high-density culture conditions of W.coagulans were optimized through single factor experiments and response surface experiments,and 10 L amplification culture was carried out under the optimal conditions.The results showed that the optimal culture conditions were culture temperature 50 ℃,initial pH 8.0,time 16 h.The optimal medium formulation was glucose 24 g/L,yeast extract powder-tryptone(1∶1)31 g/L,magnesium sulfate heptahydrate 0.29 g/L.After optimization,the OD620nm value of the strain reached 2.15,which was 68％higher than that before optimization,and the lag phase was shorter.Based on the above experimental results,the OD620nm reached 7.90 by setting the 10 L fermenter with stirring speed of 200 r/min and aeration culture.The results provided a basis for the industrial production of W.co-agulans and lay a foundation for the subsequent production of bacterial powder.
NETL
NSTL
万方数据
