凝结魏茨曼氏菌高密度培养条件优化
Optimization of high-density culture conditions for Weizmannia coagulans
伍亚龙 1杨博文 2吕鹏军 2史梅莓 2杨姗 1汪冬冬 3王勇 1张其圣4
作者信息
- 1. 四川益动源生物科技有限公司,四川 眉山 620000;四川东坡中国泡菜产业技术研究院,四川 眉山 620000
- 2. 四川省食品发酵工业研究设计院有限公司,四川 成都 611130
- 3. 四川东坡中国泡菜产业技术研究院,四川 眉山 620000
- 4. 四川东坡中国泡菜产业技术研究院,四川 眉山 620000;四川省食品发酵工业研究设计院有限公司,四川 成都 611130
- 折叠
摘要
为了满足市场对凝结魏茨曼氏菌(Weizmannia coagulans)不断增加的需求和获得高密度培养凝结魏茨曼氏菌的方法,该研究以凝结魏茨曼氏菌D1为试验对象,以MRS培养基为基础,通过单因素试验和响应面试验,对凝结魏茨曼氏菌高密度培养条件进行了优化,并在最佳条件下进行10 L放大培养.结果表明,最佳培养条件为培养温度50℃、初始pH值为8.0、培养时间16 h,最佳培养基配方为葡萄糖24 g/L;酵母浸粉-胰蛋白胨(1∶1)31 g/L、七水合硫酸镁0.29g/L.优化后菌株的OD620nm值达到了2.15,比优化前增长了68％,且延滞期更短.在上述试验结果的基础上,菌株在10 L发酵罐、搅拌速度200 r/min并通气培养的条件下,OD620nm值达到了7.90.研究结果为凝结魏茨曼氏菌的工业化生产提供了依据,为后续菌粉生产奠定基础.
Abstract
In order to meet the increasing market demand for Weizmannia coagulans and obtain a method for high-density culture of W.coagulans,using W.coagulans D1 as the experimental object,based on MRS medium,the high-density culture conditions of W.coagulans were optimized through single factor experiments and response surface experiments,and 10 L amplification culture was carried out under the optimal conditions.The results showed that the optimal culture conditions were culture temperature 50 ℃,initial pH 8.0,time 16 h.The optimal medium formulation was glucose 24 g/L,yeast extract powder-tryptone(1∶1)31 g/L,magnesium sulfate heptahydrate 0.29 g/L.After optimization,the OD620nm value of the strain reached 2.15,which was 68％higher than that before optimization,and the lag phase was shorter.Based on the above experimental results,the OD620nm reached 7.90 by setting the 10 L fermenter with stirring speed of 200 r/min and aeration culture.The results provided a basis for the industrial production of W.co-agulans and lay a foundation for the subsequent production of bacterial powder.
关键词
高密度培养/凝结魏茨曼氏菌/培养条件优化Key words
high-density culture/Weizmannia coagulans/culture conditions optimization引用本文复制引用
基金项目
四川益动源生物科技有限公司科研项目(RD001)
出版年
2024
NETL
NSTL
万方数据