摘要
为了筛选高产纤维素酶酵母菌,该研究利用孟加拉红培养基和刚果红培养基,从酱香型白酒酒醅中筛选产纤维素酶的酵母菌,通过形态学观察、生理生化试验及分子生物学技术对筛选菌株进行鉴定,并考察其耐受性并优化产酶条件.结果表明,筛选出一株产纤维素酶菌株JXJP-Y19,经鉴定后确定该菌株为解脂耶氏酵母菌(Yarrowia lipolytica).菌株JXJP-Y19的温度、乙醇、pH耐受性分别为39℃、8％(V/V)、3,整体表现出良好的环境胁迫耐受性能.最佳产酶条件为以麸皮作碳源,棉籽饼作氮源,接种量1％,初始pH值6,发酵温度30 ℃.在此优化条件下,菌株JXJP-Y19所产滤纸酶活力、外切葡聚糖酶和β-葡萄糖苷酶酶活分别为(0.913±0.025)U/mL、(1.272±0.025)U/mL、(0.937±0.030)U/mL,是优化前的2.15倍、1.81倍和1.94倍.菌株JXJP-Y19展现出良好的纤维素酶活力,可作为潜在菌株应用于纤维素生物降解利用.
Abstract
In order to screen high cellulose-producing yeast,cellulose-producing yeasts were screened from the fermented grains of sauce-flavor(Jiangxiangxing)Baijiu by Rose Bengal Agar and Congo Red Medium,and the screened strains were identified by morphological observation,physi-ological and biochemical tests,and molecular biology technique.The tolerance was investigated and the enzyme production conditions were opti-mized.The results showed that a cellulase-producing strain JXJP-Y19 was screened,and the strain JXJP-Y19 was identified as Yarrowia lipolytica.The temperature,ethanol,and pH tolerance of the strain JXJP-Y19 were 39 ℃,8％(V/V),and 3,respectively,showing good tolerance to environmental stress.The optimal enzyme production conditions were as follows:bran as carbon source,cottonseed cake as nitrogen source,inoculum 1％,initial pH 6 and fermentation temperature 30 ℃.Under these optimal conditions,the activities of filter paper enzyme,exoglucanase and β-glucosidase pro-duced by the strain JXJP-Y19 were(0.913±0.025)U/ml,(1.272±0.025)U/ml and(0.937±0.030)U/ml,respectively,which were 2.15 times,1.81 times,and 1.94 times higher than before optimization.The strain JXJP-Y 19 showed good cellulase activity and could be used as a potential strain for cellu-lose bio-degradation utilization.
基金项目
茅台学院高层次人才科研启动经费项目(mygccrc[2022]044)
茅台学院高层次人才科研启动经费项目(041)
遵义市科学技术局、茅台学院市校联合科技研发资金项目(遵市科合HZ字[2021]310号)
遵义市科学技术局、茅台学院市校联合科技研发资金项目([2022]173号)
遵义市科学技术局、茅台学院市校联合科技研发资金项目(177号)
贵州省高等学校现代白酒酿造技术工程研究中心(黔教技[2023]028号)
贵州省教育厅青年科技人才成长项目(黔教合KY字[2018]444)
维普
万方数据