摘要
该研究采用多种培养基富集方法和厌氧分离技术,从浓香型白酒窖泥中分离菌株,通过形态学观察、16SrRNA基因测序以及系统发育学分析确定其基本的分类信息,并通过气相色谱-质谱(GC-MS)及高效液相色谱(HPLC)法对颤螺旋菌科(Oscillospiraceae)己酸菌的代谢产物进行分析.结果表明,共分离得到63株细菌.基于16S rRNA基因序列可将全部菌株鉴定到属,分属于2纲3目8科15属,主要包括梭菌属(Clostridium)、植物乳植杆菌属(Lactiplantibacillus)、葡萄球菌属(Staphylococcus)、芽孢乳杆菌属(Sporolacto-bacillus)、Haloimpatiens、副梭菌属(Paraclostridium)、水原拉梅尔芽孢杆菌属(Rummeliibacillus)、产己酸菌属(Caproiciproducens)等.其中,有9株菌与近似菌的序列相似度较低(96.17％～97.70％),可能为潜在的新物种.3株颤螺旋菌科己酸菌均能利用RCM培养基发酵产乙酸、丁酸、己酸;其中Caproicibacterium sp.20-5D、Caproiciproducens sp.CPB-2和Caproiciproducens sp.YR-4的己酸产量分别为3.84 g/L、1.03 g/L和0.35g/L,具有较强的产混合酸能力.从浓香型白酒窖泥中获得的功能菌对丰富窖泥可培养微生物数量和功能微生物的应用与开发具有重要意义.
Abstract
The strains were isolated from strong-flavor(Nongxiangxing)Baijiu pit mud by a variety of medium enrichment methods and anaerobic iso-lation techniques,their basic taxonomic information was determined by morphological observation,16S rRNA gene sequencing,and phylogenetic analysis,and the metabolites of caproic acid bacteria of Oscillospiraceae were analyzed by GC-MS and HPLC.The results showed that a total of 63 strains of bacteria were isolated.Based on the 16S rRNA gene sequences,all strains could be identified to genus and belonged to 2 class,3 order,8 fami-lies and 15 genus,including Clostridium,Lactiplantibacillus,Staphylococcus,Sporolactobacillus,Haloimpatiens,Paraclostridium,Rummeliibacillus,Caproiciproducens,etc.Among them,there were 9 strains showed low sequence similarity(96.17％-97.70％)to the proximate bacteria,which might be potential new species.Both of 3 caproic acid bacteria of Oscillospiraceae was capable of producing acetic acid,butyric acid,caproic acid by fermen-tation using RCM medium,and the caproic acid production of Caproicibacterium sp.20-5D,Caproiciproducens sp.CPB-2 and Caproiciproducens sp.YR-4 was 3.84 g/L,1.03 g/L and 0.35 g/L,respectively,with strong ability of producing mixed acids.The functional bacteria isolated from the pit mud of strong-flavor Baijiu had the great significance for enriching the numbers of culturable microbes in pit mud and the application and development of functional microorganisms.
基金项目
2018年四川理工学院校级人才引进项目(2018RCL27)
泸州老窖研究生创新基金项目(UCX2022-5)
泸州老窖研究生创新基金项目(UCX2023-2)
维普
万方数据