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生姜酵素发酵工艺优化及抗氧化活性研究

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该研究以小黄姜为原料,利用乳酸菌和酵母菌混菌发酵制备生姜酵素。以接种时序、发酵温度、白砂糖添加量、发酵时间为自变量,以超氧化物歧化酶(SOD)酶活性为评价指标,采用单因素试验和Box-Behnken响应面试验对生姜酵素发酵工艺进行优化,并在最佳发酵工艺条件下,采用1,1-二苯基-2-三硝基苯肼(DPPH)、超氧阴离子自由基清除试验评价生姜酵素抗氧化能力,并对结果进行Pearson相关性分析。结果表明,生姜酵素最佳发酵工艺条件为先添加0。15%的乳酸菌,24 h后添加0。15%的酵母菌,白砂糖添加量20%,发酵温度29。5 ℃,发酵时间86 h。在此优化条件下,生姜酵素SOD酶活力为75。33 U/mL,总酚含量为0。073 mg/g。生姜酵素对DPPH、超氧阴离子自由基清除率分别为90。64%、18。69%,表明其有一定的抗氧化活性。Pearson相关性分析结果表明,总酚含量与DPPH自由基清除率、超氧阴离子自由基清除率、SOD酶活性之间呈极显著正相关(P<0。01)。
Optimization of fermentation process and antioxidant activity of ginger Jiaosu
In this study,using turmeric as raw material,the ginger Jiaosu was prepared by lactic acid bacteria and yeast mixed fermentation.The in-oculation time sequence,fermentation temperature,sugar content and fermentation time were taken as independent variables,and the activity of su-peroxide dismutase(SOD)was taken as the evaluation index,the fermentation process of ginger Jiaosu was optimized by single factor tests and Box-Behnken response surface tests.Under the optimal fermentation process conditions,the antioxidant capacity of ginger Jiaosu was evaluated by 1,1-diphenyl-2-picrylhydrazyl(DPPH)and superoxide anion radical scavenging tests.The Pearson correlation analysis was analyzed by the results.The results shown that the optimal fermentation process of ginger Jiaosu were obtained as follows:0.15%lactic acid bacteria was added first,and 0.15%yeast was added 24 h later,sugar addition 20%,fermentation temperature 29.5 ℃ and fermentation time 86 h.Under these optimal conditions,the activity of SOD was 75.33 U/ml and the total phenol content was 0.073 mg/g.The scavenging rates of DPPH and superoxide anion free radical of ginger Jiaosu were 90.64%and 18.69%,respectively,indicating that ginger Jiaosu had certain antioxidant activity.Pearson correlation analysis re-vealed that total phenol content was significantly positively correlated with DPPH free radical scavenging rates,superoxide anion radical scavenging rates and SOD enzyme activity(P<0.01).

ginger Jiaosufermentation process optimizationresponse surface methodantioxidant activitycorrelation

周灿、齐成媚、杨芳、史彩艳、王海璐、杨娜、肖新生、胡明月、胡克坚

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湖南科技学院化学与生物工程学院,湖南 永州 425199

湖南雅大智能科技有限公司,湖南 永州 425199

生姜酵素 发酵工艺优化 响应面法 抗氧化活性 相关性

2023年湖南省教育厅科学研究项目

湘教通[2023]361号23B0765

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(10)