Alleviative effect of loquat leaf extract on metabolic disorders of Drosophila melanogaster induced by high sugar diet
To investigate the alleviating effect of loquat leaf extract on metabolic disorders induced by high sugar diet,using Drosophila melanogaster as research object,D.melanogastriae was cultured with basic medium,high-sugar medium and medicated medium with 1％,5％and 10％loquat leaf extract,respectively,and their lifespan,locomotor ability,growth and developmental indices,antioxidant capacity,gut integrity and gut microbial di-versity were tested in each group.The results showed that the high-sugar diet could cause metabolic disorders in D.melanogaster,and the addition of 10％loquat leaf extract to the diet could improve the growth performance of D.melanogaster by prolonging the lifespan of D.melanogastriae,im-proving the locomotor ability of older D.melanogaster,and increasing the pupation rate and feathering rate of D.melanogaster eggs.The antioxidant capacity of D.melanogaster was improved by increasing the activities of superoxide dismutase(SOD)and catalase(CAT)in D.melanogastriae and decreasing the levels of lipid peroxide malondialdehyde(MDA).Through 16S rRNA sequencing and analysis of gut flora,it was found that high-sugar diet could lead to intestinal flora dysbiosis in D.melanogaster,and the addition of 10％loquat leaf extract to the diet could alleviate intestinal flora dysbiosis by increasing the abundance of microflora,inhibiting the proliferation of enteritis-associated microflora,and changing the composition of microflora.The results indicated that loquat leaf extract could alleviate the metabolic disorders caused by high sugar diet to a certain extent.
万方数据
维普
