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响应面法优化酒精醋发酵用营养盐配方

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为提高酒精醋的酸度及发酵效率,该研究以总酸含量为响应值,通过单因素试验结合响应面法对酒精醋发酵用营养盐配方进行了优化。结果表明,培养基中无水硫酸镁、无水氯化钙、天冬氨酸(Asp)、脯氨酸(Pro)是影响酒精醋总酸含量的主要因素。酒精醋发酵用营养盐的最佳培养基配方为:葡萄糖1。50 g/L、丙氨酸(Ala)0。15 g/L、Asp 0。44 g/L、Pro 0。18 g/L、柠檬酸铵0。15 g/L、无水氯化钙0。09 g/L、无水硫酸镁0。09 g/L、泛酸0。12 g/L。在此优化条件下,酒精醋的总酸含量为(53。85±0。22)g/L,相较优化前提升了 12。19%。
Optimization of nutrients formula for alcohol vinegar fermentation by response surface methodology
In order to improve the acidity and fermentation efficiency of alcohol vinegar,using the total acid content as the response value,the formula of nutrient salt for alcohol vinegar fermentation was optimized by single factor experiments combined with response surface method.The results showed that MgSO4,CaCl2,aspartate(Asp)and proline(Pro)were the main factors affecting the total acid content of alcohol vinegar.The optimal nutrient formula for alcohol vinegar fermentation was as follows:glucose 1.50 g/L,alanine(Ala)0.15 g/L,Asp 0.44 g/L,Pro 0.18 g/L,ammonium citrate 0.15 g/L,CaCl2 0.09 g/L,MgSO4 0.09 g/L,pantothenic acid 0.12 g/L.Under these optimal conditions,the total acid content of alcohol vinegar was(53.85±0.22)g/L,which was increased by 12.19%compared with that before optimization.

alcohol vinegartotal acid contentresponse surface methodologynutrient

宋来生、杨雅静、陈阳、洪厚胜、郭会明

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南京工业大学化学与分子工程学院,江苏南京 211816

南京工业大学生物与制药工程学院,江苏南京 211816

南京汇科生物工程设备有限公司,江苏南京 210009

酒精醋 总酸含量 响应面法 营养盐

国家高技术研究发展计划项目

2012AA021201

2024

中国酿造
中国调味品协会 北京食品科学研究院

中国酿造

CSTPCD北大核心
影响因子:0.759
ISSN:0254-5071
年,卷(期):2024.43(10)